tailieunhanh - báo cáo khoa học: "In vivo observation of gold nanoparticles in the central nervous system of Blaberus discoidalis"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: In vivo observation of gold nanoparticles in the central nervous system of Blaberus discoidalis | Rocha et al. Journal of Nanobiotechnology 2011 9 5 http content 9 1 5 JOURNAL OF NANOBIOTECHNOLOGY RESEARCH Open Access In vivo observation of gold nanoparticles in the central nervous system of Blaberus discoidalis 1 w 71 12 3 3 12 Aracely Rocha Yan Zhou Subrata Kundu Jorge M Gonzalez S BradleighVinson Hong Liang Abstract Background Nanoparticles NPs are widely studied for biomedical applications. Understanding interactions between NPs and biomolecules or cells has yet to be achieved. Here we present a novel in vivo method to study interactions between NPs and the nervous system of the discoid or false dead-head roach Blaberus discoidalis. The aims of this study were to present a new and effective method to observe NPs in vivo that opens the door to new methods of study to observe the interactions between NPs and biological systems and to present an inexpensive and easy-to-handle biological system. Results Negatively charged gold nanoparticles nAuNPs of 50 nm in diameter were injected into the central nervous system CNS of the insect. By using such a cost effective method we were able to characterize nAuNPs and to analyze their interactions with a biological system. It showed that the charged particles affected the insect s locomotion. The nAuNPs affected the insect s behavior but had no major impacts on the life expectancy of the cockroach after two months of observation. This was apparently due to the encapsulation of nAuNPs inside the insect s brain. Based on cockroach s daily activity we believed that the encapsulation occurred in the first 17 days. Conclusions The method proposed here is an inexpensive and reliable way of observing the response of biological systems to nanoparticles in-vivo. It opens new windows to further understand how nanoparticles affect neural communication by monitoring insect activity and locomotion. Background Due to their small size nanoparticles NPs have been used to probe biological systems 1-3 . .