tailieunhanh - Báo cáo khoa học: Theoretical study of lipid biosynthesis in wild-type Escherichia coli and in a protoplast-type L-form using elementary flux mode analysis

In the present study, we investigated lipid biosynthesis in the bacterium Escherichia coliby mathematical modeling. In particular, we studied the interaction between the subsystems producing unsaturated and saturated fatty acids, phospholipids, lipid A, and cardiolipin. | ỊFEBS Journal Theoretical study of lipid biosynthesis in wild-type Escherichia coli and in a protoplast-type L-form using elementary flux mode analysis Dimitar Kenanov1 f Christoph Kaleta1 z Andreas Petzold2 Christian Hoischen3 Stephan Diekmann3 Roman A. Siddiqui2 Ệ and Stefan Schuster1 1 Department of Bioinformatics Friedrich-Schiller University Jena Germany 2 Department of Genome Analysis Fritz Lipmann Institute Jena Germany 3 Department of Molecular Biology Fritz Lipmann Institute Jena Germany Keywords bacterialL-forms cell-walldeficient bacteria elementary flux modes lipid A lipid metabolism Correspondence S. Schuster Department of Bioinformatics Friedrich-Schiller University Ernst-Abbe-Platz 2 07743 Jena Germany Fax 49 3641 946452 Tel 49 3641 949580 E-mail These authors contributed equally to this work Present address fBioinformatics Institute A STAR Matrix Singapore ỊDepartment of Infection Biology Leibniz Institute for Primate Research Gottingen Germany Database Nucleotide sequence data are available in the DDBJ EMBL GenBank databases. Accession numbers are given in Doc. S2 Received 14 October 2009 revised 30 November 2009 accepted 11 December 2009 doi In the present study we investigated lipid biosynthesis in the bacterium Escherichia coli by mathematical modeling. In particular we studied the interaction between the subsystems producing unsaturated and saturated fatty acids phospholipids lipid A and cardiolipin. The present analysis was carried out both for the wild-type and for several in silico knockout mutants using the concept of elementary flux modes. Our results confirm that in the wild type there are four main products L1-phosphatidyletha-nolamine lipid A lipid A cold-adapted and cardiolipin. We found that each of these compounds is produced on several different routes indicating a high redundancy of the system under study. By analysis of the elementary flux modes remaining after the knockout

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