tailieunhanh - báo cáo khoa học: "Optimized labeling of bone marrow mesenchymal cells with superparamagnetic iron oxide nanoparticles and in vivo visualization by magnetic resonance imaging"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Optimized labeling of bone marrow mesenchymal cells with superparamagnetic iron oxide nanoparticles and in vivo visualization by magnetic resonance imaging | Jasmin et al. Journal of Nanobiotechnology 2011 9 4 http content 9 1 4 JOURNAL OF NANOBIOTECHNOLOGY RESEARCH Open Access Optimized labeling of bone marrow mesenchymal cells with superparamagnetic iron oxide nanoparticles and in vivo visualization by magnetic resonance imaging 1 1 13 Jasmin Ana Luiza M Torres Henrique MP Nunes Juliana A Passipieri Linda A Jelicks Emerson L Gasparetto4 David C Spray2 Antonio C Campos de Carvalho1 2 Rosalia Mendez-Otero1 Abstract Background Stem cell therapy has emerged as a promising addition to traditional treatments for a number of diseases. However harnessing the therapeutic potential of stem cells requires an understanding of their fate in vivo. Non-invasive cell tracking can provide knowledge about mechanisms responsible for functional improvement of host tissue. Superparamagnetic iron oxide nanoparticles SPIONs have been used to label and visualize various cell types with magnetic resonance imaging MRI . In this study we performed experiments designed to investigate the biological properties including proliferation viability and differentiation capacity of mesenchymal cells MSCs labeled with clinically approved SPIONs. Results Rat and mouse MSCs were isolated cultured and incubated with dextran-covered SPIONs ferumoxide alone or with poly-L-lysine PLL or protamine chlorhydrate for 4 or 24 hrs. Labeling efficiency was evaluated by dextran immunocytochemistry and MRI. Cell proliferation and viability were evaluated in vitro with Ki67 immunocytochemistry and live dead assays. Ferumoxide-labeled MSCs could be induced to differentiate to adipocytes osteocytes and chondrocytes. We analyzed ferumoxide retention in MSCs with or without mitomycin C pretreatment. Approximately 95 MSCs were labeled when incubated with ferumoxide for 4 or 24 hrs in the presence of PLL or protamine whereas labeling of MSCs incubated with ferumoxide alone was poor. Proliferative capacity was maintained in MSCs incubated with .