tailieunhanh - báo cáo khoa học: "Manufacturing and in vivo inner ear visualization of MRI traceable liposome nanoparticles encapsulating gadolinium"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Manufacturing and in vivo inner ear visualization of MRI traceable liposome nanoparticles encapsulating gadolinium | Zou et al. Journal of Nanobiotechnology 2010 8 32 http content 8 1 32 JOURNAL OF NANOBIOTECHNOLOGY RESEARCH Open Access Manufacturing and in vivo inner ear visualization of MRI traceable liposome nanoparticles encapsulating gadolinium I 7 1 2 2 f 1 f 3 2 1 Jing Zou Rohit Sood Sanjeev Ranjan Dennis Poe Usama A Ramadan Paavo KJ Kinnunen Ilmari Pyykko Abstract Background Treatment of inner ear diseases remains a problem because of limited passage through the blood-inner ear barriers and lack of control with the delivery of treatment agents by intravenous or oral administration. As a minimally-invasive approach intratympanic delivery of multifunctional nanoparticles MFNPs carrying genes or drugs to the inner ear is a future therapy for treating inner ear diseases including sensorineural hearing loss SNHL and Meniere s disease. In an attempt to track the dynamics and distribution of nanoparticles in vivo here we describe manufacturing MRI traceable liposome nanoparticles by encapsulating gadolinium-tetra-azacyclo-dodecane-tetra-acetic acid Gd-DOTA abbreviated as LPS Gd-DOTA and their distribution in the inner ear after either intratympanic or intracochlear administration. Results Measurements of relaxivities r1 and r2 showed that LPS Gd-DOTA had efficient visible signal characteristics for MRI. In vivo studies demonstrated that LPS Gd-DOTA with 130 nm size were efficiently taken up by the inner ear at 3 h after transtympanic injection and disappeared after 24 h. With intracochlear injection LPS Gd-DOTA were visualized to distribute throughout the inner ear including the cochlea and vestibule with fast dynamics depending on the status of the perilymph circulation. Conclusion Novel LPS Gd-DOTA were visible by MRI in the inner ear in vivo demonstrating transport from the middle ear to the inner ear and with dynamics that correlated to the status of the perilymph circulation. Background Treatments of inner ear diseases with traditional strategies