tailieunhanh - báo cáo khoa học: "Nanobiotechnology applications of reconstituted high density lipoprotein"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Nanobiotechnology applications of reconstituted high density lipoprotein | Ryan Journal of Nanobiotechnology 2010 8 28 http content 8 1 28 JOURNAL OF NANOBIOTECHNOLOGY REVIEW Open Access Nanobiotechnology applications of reconstituted high density lipoprotein Robert O Ryan1 2 Abstract High-density lipoprotein HDL plays a fundamental role in the Reverse Cholesterol Transport pathway. Prior to maturation nascent HDL exist as disk-shaped phospholipid bilayers whose perimeter is stabilized by amphipathic apolipoproteins. Methods have been developed to generate reconstituted rHDL in vitro and these particles have been used in a variety of novel ways. To differentiate between physiological HDL particles and non-natural rHDL that have been engineered to possess additional components functions the term nanodisk ND is used. In this review various applications of ND technology are described such as their use as miniature membranes for solubilization and characterization of integral membrane proteins in a native like conformation. In other work ND harboring hydrophobic biomolecules drugs have been generated and used as transport delivery vehicles. In vitro and in vivo studies show that drug loaded ND are stable and possess potent biological activity. A third application of ND is their use as a platform for incorporation of amphiphilic chelators of contrast agents such as gadolinium used in magnetic resonance imaging. Thus it is demonstrated that the basic building block of plasma HDL can be repurposed for alternate functions. Background The term high-density lipoprotein HDL describes a continuum of plasma lipoprotein particles that possess a multitude of different proteins and a range of lipid constituents 1 . The major physiological function of HDL is in Reverse Cholesterol Transport RCT 2 . The well-documented inverse relationship between plasma HDL concentration and incidence of cardiovascular disease has generated considerable interest in development of strategies to increase HDL levels. Aside from exercise moderate .