tailieunhanh - báo cáo khoa học: " Simultaneous detection of Human Immunodeficiency Virus 1 and Hepatitis B virus infections using a dual-label time-resolved fluorometric assay"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Simultaneous detection of Human Immunodeficiency Virus 1 and Hepatitis B virus infections using a dual-label time-resolved fluorometric assay | Myyrylăinen et al. Journal of Nanobiotechnology 2010 8 27 http content 8 1 27 JOURNAL OF NANOBIOTECHNOLOGY SHORT COMMUNICATION Open Access Simultaneous detection of Human Immunodeficiency Virus 1 and Hepatitis B virus infections using a dual-label time-resolved fluorometric assay 1 f 2f 2 3 1 Tiina Myyrylainen Sheikh M Talha Sathyamangalam Swaminathan Raija Vainionpaa Tero Soukka Navin Khanna2 Kim Pettersson1 Abstract A highly specific and novel dual-label time-resolved immunofluorometric assay was developed exploiting the unique emission wavelengths of the intrinsically fluorescent terbium Tb3 and europium Eu3 tracers for the simultaneous detection of human immunodeficiency virus 1 HIV-1 and hepatitis B virus HBV infections respectively. HIV-1 infection was detected using a double antigen sandwich format wherein anti-HIV-1 antibodies were captured using an in vivo biotinylated version of a chimeric HIV-1 antigen and revealed using the same antigen labeled with Tb3 chelate. Hepatitis B surface antigen HBsAg which served as the marker of HBV infection was detected in a double antibody sandwich using two monoclonal antibodies mAbs one chemically biotinylated to capture and the other labeled with Eu3 nanoparticles to reveal. The performance of the assay was evaluated using a collection n 60 of in-house and commercially available human sera panels. This evaluation showed the dual-label assay to possess high degrees of specificity and sensitivity comparable to those of commercially available single analyte-specific kits for the detection of HBsAg antigen and anti-HIV antibodies. This work demonstrates the feasibility of developing a potentially time- and resource-saving multiplex assay for screening serum samples for multiple infections in a blood bank setting. Findings The World Health Organization recommends screening for infections by human immunodeficiency virus HIV hepatitis B virus HBV hepatitis C virus HCV and Treponema pallidum .