tailieunhanh - báo cáo khoa học: "Uptake and fate of surface modified silica nanoparticles in head and neck squamous cell carcinoma"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Uptake and fate of surface modified silica nanoparticles in head and neck squamous cell carcinoma | Besic Gyenge et al. Journal of Nanobiotechnology 2011 9 32 http content 9 1 32 JOURNAL OF NANOBIOTECHNOLOGY RESEARCH Open Access Uptake and fate of surface modified silica nanoparticles in head and neck squamous cell carcinoma 1 f 1 f 2 2 3 3 Emina Besic Gyenge Xenia Darphin Amina Wirth Uwe Pieles Heinrich Walt Marius Bredell and Caroline Maake1 Abstract Background Head and neck squamous cell carcinoma HNSCC is currently the eighth leading cause of cancer death worldwide. The often severe side effects functional impairments and unfavorable cosmetic outcome of conventional therapies for HNSCC have prompted the quest for novel treatment strategies including the evaluation of nanotechnology to improve . drug delivery and cancer imaging. Although silica nanoparticles hold great promise for biomedical applications they have not yet been investigated in the context of HNSCC. In the present in-vitro study we thus analyzed the cytotoxicity uptake and intracellular fate of 200-300 nm core-shell silica nanoparticles encapsulating fluorescent dye tris bipyridine ruthenium II dichloride with hydroxyl- aminopropyl- or PEGylated surface modifications Ru@SiO2-OH Ru@SiO2-NH2 Ru@SiO2-PEG in the human HNSCC cell line UMB-sCc 745. Results We found that at concentrations of mg ml none of the nanoparticles used had a statistically significant effect on proliferation rates of UMB-SCC 745. Confocal and transmission electron microscopy showed an intracellular appearance of Ru@SiO2-OH and Ru@SiO2-NH2 within 30 min. They were internalized both as single nanoparticles presumably via clathrin-coated pits or in clusters and always localized to cytoplasmic membrane-bounded vesicles. Immunocytochemical co-localization studies indicated that only a fraction of these nanoparticles were transferred to early endosomes while the majority accumulated in large organelles. Ru@SiO2-OH and Ru@SiO2-NH2 nanoparticles had never been observed to traffic to the lysosomal .