tailieunhanh - báo cáo khoa học: "Inhibition of angiogenesis- and inflammation-inducing factors in human colon cancer cells in vitro and in ovo by free and nanoparticle-encapsulated redox dye, DCPIP"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Inhibition of angiogenesis- and inflammation-inducing factors in human colon cancer cells in vitro and in ovo by free and nanoparticle-encapsulated redox dye, DCPIP | Mondalek et al. Journal of Nanobiotechnology 2010 8 17 http content 8 1 17 RESEARCH JOURNAL OF NANOBIOTECHNOLOGY Open Access Inhibition of angiogenesis- and inflammation-inducing factors in human colon cancer cells in vitro and in ovo by free and nanoparticle-encapsulated redox dye DCPIP Fadee G Mondalek 1 4 Sivapriya Ponnurangam1 Janita Govind1 Courtney Houchen2 Shrikant Anant2 3 Panayotis Pantazis1 4 and Rama P Ramanujam1 4 Abstract Background The redox dye DCPIP has recently shown to exhibit anti-melanoma activity in vitro and in vivo. On the other hand there is increasing evidence that synthetic nanoparticles can serve as highly efficient carriers of drugs and vaccines for treatment of various diseases. These nanoparticles have shown to serve as potent tools that can increase the bioavailability of the drug vaccine by facilitating absorption or conferring sustained and improved release. Here we describe results on the effects of free- and nanoparticle-enclosed DCPIP as anti-angiogenesis and antiinflammation agents in a human colon cancer HCT116 cell line in vitro and in induced angiogenesis in ovo. Results The studies described in this report indicate that a DCPIP inhibits proliferation of HCT116 cells in vitro b DCPIP can selectively downregulate expression of the pro-angiogenesis growth factor VEGF c DCPIP inhibits activation of the transcriptional nuclear factor NF-kB d DCPIP can attenuate or completely inhibit VEGF-induced angiogenesis in the chick chorioallantoic membrane e DCPIP at concentrations higher than 6 pg ml induces apoptosis in HCT116 cells as confirmed by detection of caspase-3 and PARP degradation and f DCPIP encapsulated in nanoparticles is equally or more effective than free DCPIP in exhibiting the aforementioned properties a-e in addition to reducing the expression of COX-2 and pro-inflammatory proteins IL-6 and IL-8. Conclusions We propose that DCPIP may serve as a potent tool to prevent or disrupt the processes .