tailieunhanh - báo cáo khoa học: "Activation of stress-related signalling pathway in human cells upon SiO2 nanoparticles exposure as an early indicator of cytotoxicity"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Activation of stress-related signalling pathway in human cells upon SiO2 nanoparticles exposure as an early indicator of cytotoxicity | Mohamed et al. Journal of Nanobiotechnology 2011 9 29 http content 9 1 29 JOURNAL OF NANOBIOTECHNOLOGY RESEARCH Open Access Activation of stress-related signalling pathway in human cells upon SiO2 nanoparticles exposure as an early indicator of cytotoxicity rx I A 1 .rtl ll I 1 M I 1 A I I n A A I I tAt ll 1 A . I A fl t 1 Bashir Mustafa Mohamed Navin Kumar Verma Adriele Prina-Mello 1 Yvonne Williams Anthony M Davies s l- A u- D A 1 - . r- 1 I r -V P-A I L V Y A A r1 r I E I A 1-1 V I 1 1 I I_I nvnmAnvn-A n Cn It míũ4 I r A I I I v l 4 12 A tz-A tz-A A t P-A m A ỉ z t A4 Gabor BaKos Laragh lormey connla Edwards John Hanrahan Anna salvati Iseult Lynch Kenneth Dawson Dermot Kelleher1 and Yuri VolKov1 2 Abstract Background Nanomaterials such as SiO2 nanoparticles SiO2NP are finding increasing applications in the biomedical and biotechnological fields such as disease diagnostics imaging drug delivery food cosmetics and biosensors development. Thus a mechanistic and systematic evaluation of the potential biological and toxic effects of SiO2NP becomes crucial in order to assess their complete safe applicability limits. Results In this study human monocytic leukemia cell line THP-1 and human alveolar epithelial cell line A549 were exposed to a range of amorphous SiO2NP of various sizes and concentrations and mg ml . Key biological indicators of cellular functions including cell population density cellular morphology membrane permeability lysosomal mass pH and activation of transcription factor-2 ATF-2 were evaluated utilizing quantitative high content screening HCS approach and biochemical techniques. Despite the use of extremely high nanoparticle concentrations our findings showed a low degree of cytotoxicity within the panel of SiO2NP investigated. However at these concentrations we observed the onset of stress-related cellular response induced by SiO2NP. Interestingly cells exposed to alumina-coated SiO2NP showed low level .