tailieunhanh - báo cáo khoa học: "PVP-coated silver nanoparticles block the transmission of cell-free and cell-associated HIV-1 in human cervical culture"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: PVP-coated silver nanoparticles block the transmission of cell-free and cell-associated HIV-1 in human cervical culture | Lara et al. Journal of Nanobiotechnology 2010 8 15 http content 8 1 15 RESEARCH JOURNAL OF NANOBIOTECHNOLOGY Open Access PVP-coated silver nanoparticles block the transmission of cell-free and cell-associated HIV-1 in human cervical culture Humberto H Lara Liliana Ixtepan-Turrent Elsa N Garza-Trevino and Cristina Rodriguez-Padilla Abstract Background Previous in vitro studies have demonstrated that polyvinylpyrrolidone coated silver nanoparticles PVP-coated AgNPs have antiviral activity against HIV-1 at non-cytotoxic concentrations. These particles also demonstrate broad spectrum virucidal activity by preventing the interaction of HIV-1 gp120 and cellular CD4 thereby inhibiting fusion or entry of the virus into the host cell. In this study we evaluated the antiviral activity of PVP-coated AgNPs as a potential topical vaginal microbicide to prevent transmission of HIV-1 infection using human cervical culture an in vitro model that simulates in vivo conditions. Results When formulated into a non-spermicidal gel Replens at a concentration of mg mL PVP-coated AgNPs prevented the transmission of cell-associated HIV-1 and cell-free HIV-1 isolates. Importantly PVP-coated AgNPs were not toxic to the explant even when the cervical tissues were exposed continuously to mg mL of PVP-coated AgNPs for 48 h. Only 1 min of PVP-coated AgNPs pretreatment to the explant was required to prevent transmission of HIV-1. Pre-treatment of the cervical explant with mg mL PVP-coated AgNPs for 20 min followed by extensive washing prevented the transmission of HIV-1 in this model for 48 h. Conclusions A formulation of PVP-coated AgNPs homogenized in Replens gel acts rapidly to inhibit HIV-1 transmission after 1 min and offers long-lasting protection of the cervical tissue from infection for 48 h with no evidence of cytotoxicity observed in the explants. Based on this data PVP-coated AgNPs are a promising microbicidal candidate for use in topical .