tailieunhanh - báo cáo khoa học: "BRCAA1 monoclonal antibody conjugated fluorescent magnetic nanoparticles for in vivo targeted magnetofluorescent imaging of gastric cancer"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: BRCAA1 monoclonal antibody conjugated fluorescent magnetic nanoparticles for in vivo targeted magnetofluorescent imaging of gastric cancer | Wang et al. Journal of Nanobiotechnology 2011 9 23 http content 9 1 23 JOURNAL OF NANOBIOTECHNOLOGY RESEARCH Open Access BRCAA1 monoclonal antibody conjugated fluorescent magnetic nanoparticles for in vivo targeted magnetofluorescent imaging of gastric cancer 1 f 1f 2 1 1 1 1 Kan Wang Jing Ruan Qirong Qian Hua Song Chenchen Bao Xueqing Zhang Yifei Kong Chunlei Zhang1 Guohan Hu2 Jian Ni1 and Daxiang Cui1 Abstract Background Gastric cancer is 2th most common cancer in China and is still the second most common cause of cancer-related death in the world. How to recognize early gastric cancer cells is still a great challenge for early diagnosis and therapy of patients with gastric cancer. This study is aimed to develop one kind of multifunctional nanoprobes for in vivo targeted magnetofluorescent imaging of gastric cancer. Methods BRCAA1 monoclonal antibody was prepared was used as first antibody to stain 50 pairs of specimens of gastric cancer and control normal gastric mucous tissues and conjugated with fluorescent magnetic nanoparticles with 50 nm in diameter the resultant BRCAA1-conjugated fluorescent magnetic nanoprobes were characterized by transmission electron microscopy and photoluminescence spectrometry as-prepared nanoprobes were incubated with gastric cancer MGC803 cells and were injected into mice model loaded with gastric cancer of 5 mm in diameter via tail vein and then were imaged by fluorescence optical imaging and magnetic resonance imaging their biodistribution was investigated. The tissue slices were observed by fluorescent microscopy and the important organs such as heart lung kidney brain and liver were analyzed by hematoxylin and eosin HE stain method. Results BRCAA1 monoclonal antibody was successfully prepared BRCAA1 protein exhibited over-expression in 64 gastric cancer tissues no expression in control normal gastric mucous tissues there exists statistical difference between two groups P . The BRCAA1-conjugated .