tailieunhanh - báo cáo khoa học: " On-chip constructive cell-Network study (I): Contribution of cardiac fibroblasts to cardiomyocyte beating synchronization and community effect"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: On-chip constructive cell-Network study (I): Contribution of cardiac fibroblasts to cardiomyocyte beating synchronization and community effect | Kaneko et al. Journal of Nanobiotechnology 2011 9 21 http content 9 1 21 JOURNAL OF NANOBIOTECHNOLOGY RESEARCH Open Access On-chip constructive cell-Network study I Contribution of cardiac fibroblasts to cardiomyocyte beating synchronization and community effect Tomoyuki Kaneko Fumimasa Nomura and Kenji Yasuda Abstract Backgrounds To clarify the role of cardiac fibroblasts in beating synchronization we have made simple lined-up cardiomyocyte-fibroblast network model in an on-chip single-cell-based cultivation system. Results The synchronization phenomenon of two cardiomyocyte networks connected by fibroblasts showed 1 propagation velocity of electrophysiological signals decreased a magnitude depending on the increasing number of fibroblasts not the lengths of fibroblasts 2 fluctuation of interbeat intervals of the synchronized two cardiomyocyte network connected by fibroblasts did not always decreased and was opposite from homogeneous cardiomyocyte networks and 3 the synchronized cardiomyocytes connected by fibroblasts sometimes loses their synchronized condition and recovered to synchronized condition in which the length of asynchronized period was shorter less than 30 beats and was independent to their cultivation time whereas the length of synchronized period increased according to cultivation time. Conclusions The results indicated that fibroblasts can connect cardiomyocytes electrically but do not significantly enhance and contribute to beating interval stability and synchronization. This might also mean that an increase in the number of fibroblasts in heart tissue reduces the cardiomyocyte community effect which enhances synchronization and stability of their beating rhythms. Background Cardiomyocytes make up more than half the volume of normal heart tissue and play a role in the pumping of blood. Most of the other non-beating cells in the heart is the fibroblasts forming the cardiac skeleton and providing the mechanical scaffold .