tailieunhanh - báo cáo khoa học: "Long-term exposure of CdTe quantum dots on PC12 cellular activity and the determination of optimum non-toxic concentrations for biological use"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Long-term exposure of CdTe quantum dots on PC12 cellular activity and the determination of optimum non-toxic concentrations for biological use | Prasad et al. Journal of Nanobiotechnology 2010 8 7 http content 8 1 7 JOURNAL OF NANOBIOTECHNOLOGY RESEARCH Open Access Long-term exposure of CdTe quantum dots on PC12 cellular activity and the determination of optimum non-toxic concentrations for biological use 1 f 1 1 1 2 f 2 Babu R Prasad Natalia Nikolskaya David Connolly Terry J Smith Stephen J Byrne Valérie A Gérard Yurii K Gun ko2 Yury Rochev1 Abstract Background The unique and tuneable photonic properties of Quantum Dots QDs have made them potentially useful tools for imaging biological entities. However QDs though attractive diagnostic and therapeutic tools have a major disadvantage due to their inherent cytotoxic nature. The cellular interaction uptake and resultant toxic influence of CdTe QDs gelatinised and non-gelatinised Thioglycolic acid TGA capped have been investigated with pheochromocytoma 12 PC12 cells. In conjunction to their analysis by confocal microscopy the QD - cell interplay was explored as the QD concentrations were varied over extended up to 72 hours co-incubation times. Coupled to this investigation cell viability DNA quantification and cell proliferation assays were also performed to compare and contrast the various factors leading to cell stress and ultimately death. Results Thioglycolic acid TGA stabilised CdTe QDs gel and non - gel were co-incubated with PC12 cells and investigated as to how their presence influenced cell behaviour and function. Cell morphology was analysed as the QD concentrations were varied over co-incubations up to 72 hours. The QDs were found to be excellent fluorophores illuminating the cytoplasm of the cells and no deleterious effects were witnessed at concentrations of 10-9 M. Three assays were utilised to probe how individual cell functions viability DNA quantification and proliferation were affected by the presence of the QDs at various concentrations and incubation times. Cell response was found to not only be concentration .