tailieunhanh - báo cáo khoa học: "Co-transfection of plasmid DNA and laser-generated gold nanoparticles does not disturb the bioactivity of GFP-HMGB1 fusion protein"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Co-transfection of plasmid DNA and laser-generated gold nanoparticles does not disturb the bioactivity of GFP-HMGB1 fusion protein | Journal of Nanobiotechnology BioMed Central Research Co-transfection of plasmid DNA and laser-generated gold nanoparticles does not disturb the bioactivity of GFP-HMGB1 fusion protein Svea Petersen 1 Jan T Soiled2 3 Siegfried Wagner2 3 Andreas Richter3 Jorn Bullerdiek2 3 Ingo Nolte2 Stephan Barcikowski 1 and Hugo Murua Escobar2 Open Access Address 1Laser Zentrum Hannover . Hannover Germany 2Small Animal Clinic and Research Cluster of Excellence REBIRTH University of Veterinary Medicine Bischofsholer Damm 15 D-30173 Hannover Germany and 3Centre for Human Genetics University of Bremen Leobener Strasse zHg D-28359 Bremen Germany Email Svea Petersen - Jan T Soller - Siegfried Wagner - Andreas Richter - Jorn Bullerdiek - bullerd@ Ingo Nolte - Stephan Barcikowski - Hugo Murua Escobar - hescobar@ Corresponding author fEqual contributors Published 24 October 2009 Received 27 March 2009 Accepted 24 October 2009 Journal of Nanobiotechnology 2009 7 6 doi 1477-3155-7-6 This article is available from http content 7 1 6 2009 Petersen et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Ultrashort pulsed laser ablation in liquids represents a powerful tool for the generation of pure gold nanoparticles AuNPs avoiding chemical precursors and thereby making them especially interesting for biomedical applications. However because of their electron accepting properties laser-generated AuNPs might affect biochemical properties of biomolecules which often adsorb onto the nanoparticles. We investigated possible effects of