tailieunhanh - báo cáo khoa học: "Formulation of polylactide-co-glycolic acid nanospheres for encapsulation and sustained release of poly(ethylene imine)-poly(ethylene glycol) copolymers complexed to oligonucleotides"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Formulation of polylactide-co-glycolic acid nanospheres for encapsulation and sustained release of poly(ethylene imine)-poly(ethylene glycol) copolymers complexed to oligonucleotides | Journal of Nanobiotechnology BioMed Central Research Formulation of polylactide-co-glycolic acid nanospheres for encapsulation and sustained release of poly ethylene imine -poly ethylene glycol copolymers complexed to oligonucleotides Shashank R Sirsi2 Rebecca C Schray1 Margaret A Wheatley2 and Gordon J Lutz 1 Open Access Address 1Drexel University College of Medicine Department of Pharmacology and Physiology Philadelphia Pennsylvania 19102 USA and 2Drexel University School of Biomedical Engineering Philadelphia Pennsylvania 19104 USA Email Shashank R Sirsi - srs43@ Rebecca C Schray - rcs46@ MargaretAWheatley-wheatley@ Gordon J Lutz - glutz@ Corresponding author Published 7 April 2009 Received 27 December 2008 Accepted 7 April 2009 Journal of Nanobiotechnology 2009 7 1 doi 1477-3155-7-1 This article is available from http content 7 1 1 2009 Sirsi et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Antisense oligonucleotides AOs have been shown to induce dystrophin expression in muscles cells of patients with Duchenne Muscular Dystrophy DMD and in the mdx mouse the murine model of DMD. However ineffective delivery of AOs limits their therapeutic potential. Copolymers of cationic poly ethylene imine PEI and non-ionic poly ethylene glycol PEG form stable nanoparticles when complexed with AOs but the positive surface charge on the resultant PEG-PEI-AO nanoparticles limits their biodistribution. We adapted a modified double emulsion procedure for encapsulating PEG-PEI-AO polyplexes into degradable polylactide-co-glycolic acid PLGA nanospheres. Formulation parameters were varied including PLGA molecular weight ester end-capping and .