tailieunhanh - báo cáo khoa học: " Three-dimensional reconstruction of cell nuclei, internalized quantum dots and sites of lipid peroxidation"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Three-dimensional reconstruction of cell nuclei, internalized quantum dots and sites of lipid peroxidation | Journal of Nanobiotechnology BioMed Central Research Open Access Three-dimensional reconstruction of cell nuclei internalized quantum dots and sites of lipid peroxidation W Robert J FunnelW1 and Dusica Maysinger t2 Address departments of BioMedical Engineering and Otolaryngology McGill University 3775 rue University Montréal QC H3A 2B4 Canada and department of Pharmacology Therapeutics McGill University 3655 promenade Sir-William-Osler Montréal QC H3G 1Y6 Canada Email W Robert J Funnell - Dusica Maysinger - Corresponding authors tEqual contributors Published 20 October 2006 Received 17 August 2006 Accepted 20 October 2006 Journal of Nanobiotechnology 2006 4 10 doi l477-3l55-4-l 0 r This article is available from http content 4 l l0 2006 Funnell and Maysinger licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background The purpose of the study was to develop and illustrate three-dimensional 3-D reconstruction of nuclei and intracellular lipid peroxidation in cells exposed to oxidative stress induced by quantum dots. Programmed cell death is characterized by multiple biochemical and morphological changes in different organelles including nuclei mitochondria and lysosomes. It is the dynamics of the spatio-temporal changes in the signalling and morphological adaptations which will ultimately determine the shape and fate of the cell. Results We present new approaches to the 3-D reconstruction of organelle morphology and biochemical changes in confocal live-cell images. We demonstrate the 3-D shapes of nuclei the 3D intracellular distributions of QDs and the accompanying lipid-membrane peroxidation and provide methods for quantification.