tailieunhanh - báo cáo khoa học: "A nanoparticle-based immobilization assay for prion-kinetics study"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: A nanoparticle-based immobilization assay for prion-kinetics study | Journal of Nanobiotechnology BioMed Central Research A nanoparticle-based immobilization assay for prion-kinetics study Gilles K Kouassi 1 and Joseph Irudayaraj2 Open Access Address Agricultural and Biological Engineering The Pennsylvania State University State College University Park PA 16802 US and 2Bindley Biosciences Center Purdue University 225 S. University St. West Lafayette Indiana 47907 US Email Gilles K Kouassi - gkk2@ Joseph Irudayaraj- josephi@ Corresponding author Published 17 August 2006 Received 03 November 2005 . rr . Accepted 17 August 2006 Journal of Nanobiotechnology 2006 4 8 doi l477-3l55-4-8 This article is available from http content 4 l 8 2006 Kouassi and Irudayaraj licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract_ Magnetic and gold coated magnetic nanoparticles were synthesized by co-precipitation of ferrous and ferric chlorides and by the micromicelles method respectively. Synthesized nanoparticles were functionalized to bear carboxyl and amino acid moieties and used as prion protein carriers after carbodiimide activation in the presence of N-hydroxysuccinimide. The binding of human recombinant prion protein huPrPrec to the surface of these nanoparticles was confirmed by FTIR and the size and structures of the particles were characterized by transmission electron microscopy. Findings indicate that the rate of prion binding increased only slightly when the concentration of prion in the reaction medium was increased. Rate constants of binding were very similar on Fe3O4@Au and Fe3O4-LAA when the concentrations of protein were l 2 and pg ml. For a 5 pg ml .

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