tailieunhanh - báo cáo khoa học: "Phagocytic response to fully controlled plural stimulation of antigens on macrophage using on-chip microcultivation system"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Phagocytic response to fully controlled plural stimulation of antigens on macrophage using on-chip microcultivation system | Journal of Nanobiotechnology BioMed Central Short Communication Open Access Phagocytic response to fully controlled plural stimulation of antigens on macrophage using on-chip microcultivation system Kazunori Matsumura1 Kazuki Orita1 Yuichi Wakamoto1 and Kenji Yasuda 1 2 Address Department of Life Sciences Graduate school of Arts and Sciences The University of Tokyo 3-8-1 Komaba Meguro Tokyo 153-8902 Japan and 2Division of Biosystems Institute of Biomaterials and Bioengineering Tokyo Medical and Dental University 2-3-10 Kanda-Surugadai Chiyoda-ku Tokyo 101-0062 Japan Email Kazunori Matsumura - matsumura_kazunori@ Kazuki Orita - orita_kazuki@ Yuichi Wakamoto - wakamoto_yuichi@ Kenji Yasuda - cyasuda@ Corresponding author Published 16 August 2006 Received 27 March 2006 . Accepted 16 August 2006 Journal of Nanobiotechnology 2006 4 7 doi l477-3l55-4-7 This article is available from http content 4 l 7 2006 Matsumura et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract To understand the control mechanism of innate immune response in macrophages a series of phagocytic responses to plural stimulation of antigens on identical cells was observed. Two zymosan particles which were used as antigens were put on different surfaces of a macrophage using optical tweezers in an on-chip single-cell cultivation system which maintains isolated conditions of each macrophage during their cultivation. When the two zymosan particles were attached to the macrophage simultaneously the macrophage responded and phagocytosed both of the antigens simultaneously. In contrast when the second antigen was attached to the surface after .