tailieunhanh - báo cáo khoa học: "Quantum dots – a versatile tool in plant science?"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Quantum dots – a versatile tool in plant science? | Journal of Nanobiotechnology BioMed Central Short Communication Open Access Quantum dots - a versatile tool in plant science Frank Muller1 2 Andreas Houben1 Peter E Barker3 Yan Xiao3 Josef A Kas2 and Michael Melzer 1 Address 1Institute of Plant Genetics and Crop Plant Research IPK 06466 Gatersleben Germany 2University of Leipzig Faculty of Physics and Geosciences Leipzig Germany and 3National Institute of Standards and Technology Gaithersburg Maryland 20899 USA Email Frank Muller - Andreas Houben - houben@ Peter E Barker - Yan Xiao - Josef A Kas - jkaes@ Michael Melzer - melzer@ Corresponding author Published 15 June 2006 Received 10 February 2006 . r . rr . r Accepted 15 June 2006 Journal of Nanobiotechnology 2006 4 5 doi 1477-3155-4-5 This article is available from http content 4 1 5 2006 Muller et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract An optically stable novel class of fluorophores quantum dots for in situ hybridisation analysis was tested to investigate their signal stability and intensity in plant chromosome analyses. Detection of hybridisation sites in situ was based on fluorescence from streptavidin-linked inorganic crystals of cadmium selenide. Comparison of quantum dots QDs with conventional detection systems Alexa 488 in immunolabeling experiments demonstrated greater sensitivity than the conventional system. In contrast detection of QDs in in situ hybridisation of several plant chromosomes using several high-copy sequences was less sensitve than Alexa 488. Thus semiconductor nanocrystal fluorophores are more suitable for .