tailieunhanh - Báo cáo y học: " Increased expression of matrix metalloproteinase-10, nerve growth factor and substance P in the painful degenerate intervertebral disc"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Increased expression of matrix metalloproteinase-10, nerve growth factor and substance P in the painful degenerate intervertebral disc. | Available online http content 11 4 R126 Research article Increased expression of matrix metalloproteinase-10 nerve growth factor and substance P in the painful degenerate intervertebral disc Stephen M Richardson1 Paul Doyle2 Ben M Minogue1 Kanna Gnanalingham2 and Judith A Hoyland1 Tissue Injury and Repair Group School of Clinical and Laboratory Sciences Stopford Building The University of Manchester Oxford Road Manchester M13 9pT UK 2Department of Neurosurgery Greater Manchester Neuroscience Centre Salford Royal Foundation Trust Stott Lane Salford M6 8HD UK Corresponding author Judith A Hoyland Received 9 Jun 2009 Revisions requested 8 Jul 2009 Revisions received 27 Jul 2009 Accepted 20 Aug 2009 Published 20 Aug 2009 Arthritis Research Therapy 2009 11 R126 doi 86 ar2793 This article is online at http content 11 4 R126 2009 Richardson et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Open Access Abstract Introduction Matrix metalloproteinases MMPs are known to be involved in the degradation of the nucleus pulposus NP during intervertebral disc IVD degeneration. This study investigated MMP-10 stromelysin-2 expression in the NP during IVD degeneration and correlated its expression with pro-inflammatory cytokines and molecules involved in innervation and nociception during degeneration which results in low back pain LBP . Methods Human NP tissue was obtained at postmortem PM from patients without a history of back pain and graded as histologically normal or degenerate. Symptomatic degenerate NP samples were also obtained at surgery for LBP. Expression of MMP-10 mRNA and protein was analysed using real-time polymerase chain reaction and .