tailieunhanh - Báo cáo y học: " AIT test has no problem in the detection of anti-ribosomal P – authors’ response"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: AIT test has no problem in the detection of anti-ribosomal P – authors’ response. | Available online http content 11 4 411 Letter AIT test has no problem in the detection of anti-ribosomal P -authors response Michael Mahler1 and Marvin J Fritzler2 1Dr Fooke Laboratorien GmbH MainstraBe 85 41469 Neuss Germany 2Faculty of Medicine HRB410B University of Calgary 3330 Hospital Dr NW Calgary Alberta Canada T2N 4N1 Corresponding author Marvin J Fritzler fritzler@ Published 25 August 2009 Arthritis Research Therapy 2009 11 411 doi ar2776 This article is online at http content 11 4 411 2009 BioMed Central Ltd See related letter by Jearn and Kim http content 11 3 407 and related research by Mahler et al. http content 10 6 R131 In a recent article Jearn and Kim assessed the ability of indirect immunofluorescence on a novel human macrophage cell line IT-1 in an autoimmune target AIT assay to predict the presence of anti-ribosomal P anti-Rib-P antibodies 1 based on a previously described cytoplasmic and or nucleolar staining pattern 2 . Despite the clearance of the AIT test by the South Korea Food and Drug Administration to the best of our knowledge this test is not widely used as a screening method - neither is it approved by the Food and Drug Administration FDA USA or is it a Communauté Européenne CE certified method for the detection of antinuclear antibodies. By contrast many laboratories rely on HEp-2 cell substrates that are widely available as FDA and CE approved diagnostic kits from a variety of manufacturers. In their study Jearn and Kim identified sera with anti-Rib-P reactivity based on a double immunodiffusion ID assay and then used these sera to determine the positivity and staining patterns in the AIT test. This method raises concerns because it is generally known that immunodiffusion is a relatively insensitive technique for the detection of human autoantibodies when compared with other techniques such as ELISA and western .

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