tailieunhanh - Báo cáo y học: "Flow cytometry analysis of glucocorticoid receptor expression and binding in steroid-sensitive and steroid-resistant patients with systemic lupus erythematosus"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Flow cytometry analysis of glucocorticoid receptor expression and binding in steroid-sensitive and steroid-resistant patients with systemic lupus erythematosus. | Available online http content 11 4 R108 Research article Flow cytometry analysis of glucocorticoid receptor expression and binding in steroid-sensitive and steroid-resistant patients with systemic lupus erythematosus Juan Du1 Min Li2 Denghai Zhang1 Xiaoyan Zhu3 Weiwei Zhang4 Wei Gu1 Yinglu Feng1 Xiaofeng Zhai1 and Changquan Ling1 Department of Integrative Medicine Changhai Hospital The Second Military Medicine University Changhai Road Shanghai PR China 2Department of Naval Medicine The Second Military Medicine University Xiangyin Road Shanghai PR China 3Department of physiology The Second Military Medicine University Xiangyin Road Shanghai PR China 4Department of Medical Technology Changhai Hospital The Second Military Medicine University Changhai Road Shanghai PR China Contributed equally Corresponding author Changquan Ling lingchangquan@ Received 9 Jan 2009 Revisions requested 9 Feb 2009 Revisions received 9 May 2009 Accepted 14 Jul 2009 Published 14 Jul 2009 Arthritis Research Therapy 2009 11 R108 doi 86 ar2763 This article is online at http content 11 4 R108 2009 Du et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Open Access Abstract Introduction Glucocorticoid GC therapy is the main treatment for systemic lupus erythematosus SLE . However some patients are resistant to these agents. Abnormalities of glucocorticoid receptor GR seem to be related to steroid resistance. This study evaluated GRs in T lymphocytes and monocytes of SLE patients by flow cytometry FCM using a monoclonal antibody mAb and FITC-Dex probes. Methods Thirty-five patients with SLE before treatment and 27 age- and sex-matched normal controls were studied.