tailieunhanh - Báo cáo y học: "Hypertrophy is induced during the in vitro chondrogenic differentiation of human mesenchymal stem cells by bone morphogenetic protein-2 and bone morphogenetic protein-4 gene transfer"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Hypertrophy is induced during the in vitro chondrogenic differentiation of human mesenchymal stem cells by bone morphogenetic protein-2 and bone morphogenetic protein-4 gene transfer. | Available online http content 11 5 R148 Research article Hypertrophy is induced during the in vitro chondrogenic differentiation of human mesenchymal stem cells by bone morphogenetic protein-2 and bone morphogenetic protein-4 gene transfer Andre F Steinert1 2 Benedikt Proffen1 Manuela Kunz1 Christian Hendrich1 Steven C Ghivizzani2 3 Ulrich Noth1 Axel Rethwilm4 Jochen Eulert1 and Christopher H Evans2 Orthopaedic Center for Musculoskeletal Research Orthopaedic Clinic Konig-Ludwig-Haus Julius-Maximilians-University Brettreichstrasse 11 97074 Wurzburg Germany 2Center for Molecular Orthopaedics Harvard Medical School 221 Longwood Avenue BLI 152 Boston MA 02115 USA 3Department of Orthopaedics and Rehabilitation University of Florida 3450 Hull Road Gainesville FL 32607 USA 4Institut fur Virologie und Immunbiologie Julius-Maximilians-University Versbacherstrasse 7 97078 Wurzburg Germany Corresponding author Christopher H Evans cevans@ Received 1 May 2009 Revisions requested 10 Jun 2009 Revisions received 15 Sep 2009 Accepted 2 Oct 2009 Published 2 Oct 2009 Arthritis Research Therapy 2009 11 R148 doi 86 ar2822 This article is online at http content 11 5 R148 2009 Steinert et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Open Access Abstract Introduction The present study compares bone morphogenetic protein BMP -4 and BMP-2 gene transfer as agents of chondrogenesis and hypertrophy in human primary mesenchymal stem cells MSCs maintained as pellet cultures. Methods Adenoviral vectors carrying cDNA encoding human BMP-4 were constructed by cre-lox combination and compared to previously generated adenoviral vectors for BMP-2 green fluorescent .