tailieunhanh - Báo cáo y học: "Synovial fluid level of aggrecan ARGS fragments is a more sensitive marker of joint disease than glycosaminoglycan or aggrecan levels: a cross-sectional study"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Synovial fluid level of aggrecan ARGS fragments is a more sensitive marker of joint disease than glycosaminoglycan or aggrecan levels: a cross-sectional study. | Available online http content 11 3 R92 Research article Synovial fluid level of aggrecan ARGS fragments is a more sensitive marker of joint disease than glycosaminoglycan or aggrecan levels a cross-sectional study Staffan Larsson L Stefan Lohmander and André Struglics Open Access Department of Orthopaedics Clinical Sciences Lund Lund University SE-221 85 Lund Sweden Corresponding author Staffan Larsson Received 8 Feb 2009 Revisions requested 16 Mar 2009 Revisions received 19 May 2009 Accepted 22 Jun 2009 Published 22 Jun 2009 Arthritis Research Therapy 2009 11 R92 doi ar2735 This article is online at http content 11 3 R92 2009 Larsson et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Introduction Aggrecanase cleavage at the 392Glu-393Ala bond in the interglobular domain IGD of aggrecan releasing N-terminal 393ARGS fragments is an early key event in arthritis and joint injuries. Here we use a quantitative immunoassay of aggrecan ARGS neoepitope fragments in human synovial fluid to determine if this cleavage-site specific method better identifies joint pathology than previously available less specific aggrecan assays. Methods Synovial fluid SF from 26 people with healthy knees reference and 269 patients were analyzed in a cross-sectional study. Patient groups were acute inflammatory arthritis acute knee injury chronic knee injury and knee osteoarthritis OA . Aggrecan ARGS fragments were assayed by ELISA using the monoclonal antibody OA-1. Total aggrecan content was analyzed by an ELISA using the monoclonal antibody 1-F21 and sulfated glycosaminoglycan by Alcian blue precipitation. Results Aggrecan ARGS fragment concentrations in all