tailieunhanh - DNA Replication Methods and Protocols

DNA replication is fundamental to cellular life on earth, and replication initiation provides the primary point of control over this process. Replication initiation in all organisms involves the interaction of initiator proteins with one or more origins of replication in the DNA, with subsequent regulated assembly of two replisome complexes at each origin, melting of the DNA, and primed initiation of DNA synthesis on leading and lagging strands. Archaea and Eukarya share homologous systems for DNA replication initiation, but differ in the complexity of these; Bacteria appear to have analogous, rather than homologous, mechanisms for replication initiation. This chapter provides an overview of current knowledge of initiation. | Springer Protocols Methods in Molecular Biology 521 DrtA Replication Methods and Protocols Humana Press Methods in Molecular Biology Series Editor John M. Walker School of Life Sciences University of Hertfordshire Hatfield Hertfordshire AL10 9AB UK For other titles published in this series go to series 521 DNA Replication Methods and Protocols Edited by Sonya Vengrova and Jacob Z. Dalgaard Marie Curie Research Institute The Chart Oxted Surrey UK Humana .