tailieunhanh - Báo cáo y học: "Upregulated miR-146a expression in peripheral blood mononuclear cells from rheumatoid arthritis patients"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Upregulated miR-146a expression in peripheral blood mononuclear cells from rheumatoid arthritis patients. | Available online http content 10 4 R101 Open Access Research article Upregulated miR-146a expression in peripheral blood mononuclear cells from rheumatoid arthritis patients Kaleb M Pauley1 Minoru Satoh2 3 Annie L Chan2 Michael R Bubb2 Westley H Reeves2 3 and Edward KL Chan1 Department of Oral Biology University of Florida SW Archer Road Gainesville Florida 32610 USA division of Rheumatology and Clinical Immunology Department of Medicine University of Florida SW Archer Road Gainesville Florida 3261 0 USA department of Pathology Immunology and Laboratory Medicine University of Florida Gainesville SW Archer Road Florida 32610 USA Corresponding author Edward KL Chan echan@ Received 29 May 2008 Revisions requested 26 Jun 2008 Revisions received 8 Aug 2008 Accepted 29 Aug 2008 Published 29 Aug 2008 Arthritis Research Therapy 2008 10 R101 doi ar2493 This article is online at http content 10 4 R101 2008 Pauley et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Introduction MicroRNAs are small noncoding RNA molecules that negatively regulate gene expression via degradation or translational repression of their targeted mRNAs. It is known that aberrant microRNA expression can play important roles in cancer but the role of microRNAs in autoimmune diseases is only beginning to emerge. In this study the expression of selected microRNAs is examined in rheumatoid arthritis. Methods Total RNA was isolated from peripheral blood mononuclear cells obtained from patients with rheumatoid arthritis and healthy and disease control individuals and the expression of miR-146a miR-155 miR-132 miR-16 and microRNA let-7a was analyzed using quantitative real-time PCR. Results .