tailieunhanh - Báo cáo y học: "Induction of HLA-B27 heavy chain homodimer formation after activation in dendritic cells"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Induction of HLA-B27 heavy chain homodimer formation after activation in dendritic cells. | Available online http content 10 4 R100 Research article Induction of HLA-B27 heavy chain homodimer formation after activation in dendritic cells Susana G Santos1 Sarah Lynch1 Elaine C Campbell1 Antony N Antoniou2 and Simon J Powis1 1Bute Medical School Westburn Lane University of St Andrews Fife KY16 9TS UK 2Department of Immunology and Molecular Pathology Windeyer Institute of Medical Science 46 Cleveland St University College London London W1T 4JF UK Corresponding author Simon J Powis sjp10@ Received 17 Apr 2008 Revisions requested 10 Jun 2008 Revisions received 24 Jul 2008 Accepted 29 Aug 2008 Published 29 Aug 2008 Arthritis Research Therapy 2008 10 R100 doi ar2492 This article is online at http content 10 4 R100 2008 Santos et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Open Access Abstract Introduction Ankylosing spondylitis AS is a severe chronic inflammatory arthritis with a strong association to the human major histocompatibilty complex MHC class I allele human leucocyte antigen HLA B27. Disulfide-linked HLA-B27 heavychain homodimers have been implicated as novel structures involved in the aetiology of AS. We have studied the formation of HLA-B27 heavy-chain homodimers in human dendritic cells which are key antigen-presenting cells and regulators of mammalian immune responses. Method Both an in vitro dendritic-like cell line and monocyte-derived dendritic cells from peripheral blood were studied. The KG-1 dendritic-like cell line was transfected with HLA-B27 cDNA constructs and the cellular distribution intracellular assembly and ability of HLA-B27 to form heavy-chain homodimers was compared with human monocyte-derived dendritic cells .