tailieunhanh - Báo cáo y học: "Monocytes are essential for inhibition of synovial T-cell glucocorticoid-mediated apoptosis in rheumatoid arthritis"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Monocytes are essential for inhibition of synovial T-cell glucocorticoid-mediated apoptosis in rheumatoid arthritis. | Available online http content 10 6 R147 Research article Monocytes are essential for inhibition of synovial T-cell glucocorticoid-mediated apoptosis in rheumatoid arthritis Dimitrios Makrygiannakis Shankar Revu Petra Neregârd Erik af Klint Omri Snir Cecilia Grundtman and Anca Irinel Catrina Open Access Department of Rheumatology Karolinska University Hospital and Karolinska Institutet Stockholm S-17176 Sweden Corresponding author Anca Irinel Catrina Received 14 Jul 2008 Revisions requested 21 Aug 2008 Revisions received 22 Nov 2008 Accepted 19 Dec 2008 Published 1 9 Dec 2008 Arthritis Research Therapy 2008 10 R147 doi ar2582 This article is online at http content 10 6 R147 2008 Makrygiannakis et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Introduction Rheumatoid arthritis RA is characterized by synovial inflammation with local accumulation of mononuclear cells such as macrophages and lymphocytes. We previously demonstrated that intra-articular glucocorticoids decrease the synovial tissue ST T-cell population and therefore aimed to investigate whether this is mediated through modulation of apoptosis. Methods Apoptosis and cell phenotype were evaluated by immunohistochemistry and dual-immunofluorescence in synovial biopsy sections from 12 RA patients before and after a mean of 11 days of an intra-articular triamcinolone knee injection. In vitro RA synovial fluid SF -derived T cells were evaluated for Annexin V expression by multicolor flow cytometry after 24-hour exposure to dexamethasone methylprednisolone or triamcinolone. We also tested induction of apoptosis by dexamethasone on psoriatic arthritis SF-derived T cells using the same .

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