tailieunhanh - Báo cáo khoa học: Prevalence of intrinsic disorder in the hepatitis C virus ARFP/Core+1/S protein

The hepatitis C virus (HCV) Core+1/S polypeptide, also known as alter-native reading frame protein (ARFP)/S, is an ARFP expressed from the Core coding region of the viral genome. Core+1/S is expressed as a result of internal initiation at AUG codons (85–87) located downstream of the polyprotein initiator codon, and corresponds to the C-terminal part of most ARFPs. | Prevalence of intrinsic disorder in the hepatitis C virus ARFP Core 1 S protein Anissa Boumlic1 2 Yves Nominé1 Sebastian Charbonnier1 Georgia Dalagiorgou2 Niki Vassilaki2 Bruno Kieffer3 Gilles Trave1 Penelope Mavromara2 and Georges Orfanoudakis1 1 Oncoproteins Group Universite de Strasbourg CNRS FRE 3211 Ecole Superieure de Biotechnologie de Strasbourg Illkirch France 2 Molecular Virology Laboratory Hellenic Pasteur Institute Athens Greece 3 Biomolecular NMR Group UMR CNRS 7104 Institut de Genetique et de Biologie Moleculaire et Cellulaire Illkirch France Keywords ARFP Core 1 S hepatitis C virus HCV intrinsic disorder IUP IDP NMR Correspondence G. Orfanoudakis Oncoproteins Group Universite de Strasbourg CNRS FRE 3211 Ecole Superieure de Biotechnologie de Strasbourg Illkirch France Fax 33 3 68 85 47 70 Tel 33 3 68 85 47 65 E-mail These authors contributed equally to this work Received 25 October 2009 revised 30 November 2009 accepted 1 December 2009 doi The hepatitis C virus HCV Core 1 S polypeptide also known as alternative reading frame protein ARFP S is an ARFP expressed from the Core coding region of the viral genome. Core 1 S is expressed as a result of internal initiation at AUG codons 85-87 located downstream of the polyprotein initiator codon and corresponds to the C-terminal part of most ARFPs. Core 1 S is a highly basic polypeptide and its function still remains unclear. In this work untagged recombinant Core 1 S was expressed and purified from Escherichia coli in native conditions and was shown to react with sera of HCV-positive patients. We subsequently undertook the biochemical and biophysical characterization of Core 1 S. The conformation and oligomeric state of Core 1 S were investigated using size exclusion chromatography dynamic light scattering fluorescence CD and NMR. Consistent with sequence-based disorder predictions Core 1 S lacks significant secondary structure in vitro which .

• Báo cáo khoa học
• Scientific reports
• scientific research
• biochemistry
• cell structure
• Medical Research
• chemical reaction
• Elements of Chemical Reaction Engineering
• Chemical Reaction Engineering
• Hóa học và ứng dụng
• Kỹ thuật hóa học
• Phản ứng hóa học
• Kỹ thuật phản ứng hóa học
• The chemical vapor deposition condition
• The electrochemically catalytic efficiency
• Hydrogen evolution reaction in MoS2 nanoparticles
• Hydrogen evolution reaction
• Metal organic chemical vapor deposition
• Reaction Kinetics
• Heterogeneous Catalysis
• Elementary Reactions
• Microkinetic Analysis
• Nonisothermal Reactors
• Catalytic Reactions
• Quantum chemical modeling mechanism
• Semiempirical method RM1
• Quantum chemical study
• Half chair shape
• Diels Alder reaction
• Chemical reaction engirneering
• Hoá phân tích
• Giải pháp phản ứng hóa học
• Radiation on MHD casson fluid flow
• Chemical reaction effects on MHD casson fluid flow
• Porous medium with suction injection
• The occurrence of suction/injection
• The central PDEs
• Radiation of MHD visco elastic fluid flow over
• Chemical reaction on heat of MHD visco elastic
• Mass transfer of MHD visco elastic fluid flow
• Exponentially stretching sheet through porous medium
• Radiation on the boundary layer MHD casson fluid
• Chemical reaction effects on the boundary layer MHD casson fluid
• Vertical plate embedded in the porous medium
• Casson fluid flow free convection boundary layer fl
• Extreme learning machine
• Artificial chemical reaction optimization algorithm
• Single hidden layer feed forward neural network
• Learning algorithm
• Chemical reaction kinetic
• Adsorption kinetics
• Pseudo first order model
• Pseudo second order model
• General kinetic model
• Dried Probiotics
• Industrial Processes
• Membrane Separation
• Wastewater
• Maillard Reaction
• Pros and Cons
• Chemical kinetics
• Reactant concentration
• Reaction rates and stoichiometry
• Action of catalysts
• Surface area
• Chemical kinetics
• Introduction to general
• Chemical bonds
• Chemical reactions
• Reaction rates
• Chemical equilibrium
• Nuclear chemistry
• Nuclear engineering and technology
• Correlation between rare earth elements
• The chemical interactions of HT9 cladding
• FueleCladding Chemical Interaction
• Higher reaction thickness
• Summary of Chemical PhD Thesis
• Theoretical and Physical Chemistry
• CO hydrogenation reaction
• Ni Cu bimetallic catalyst system
• Density functional method
• Chemical method
• Lecture General Chemistry 1
• Bài giảng Hóa học đại cương 1
• Balance chemical equations
• Calculate the percent yield of a reaction
• Chemical formulas
• Chemical equations
• organic chemistry
• hóa hữu cơ
• công nghệ hóa học
• Chemical Engineering
• common reaction mechanisms
• Chromatography in the petroleum industry
• Report medicine
• traditional medicine
• oxidation
• tumor cells
• BMC Bioinformatics
• Flux balance analysis
• Linear programming
• Design of metabolic network
• Constraint based model
• Growth rate
• Production rate
• Smaller reaction network
• Luận án Tiến sĩ
• Physical chemistry
• Fabbricated electrocatalysts
• Oxygen evolution reaction
• Proton exchange membrane
• breast cancer
• medicine
• cytotoxicity
• Atomic nucleus
• electrons
• Chemical compounds
• Electrochemistry