tailieunhanh - Báo cáo khoa học: Measuring enzyme activities under standardized in vivo-like conditions for systems biology

Realistic quantitative models require data from many laboratories. There-fore, standardization of experimental systems and assay conditions is crucial. Moreover, standards should be representative of thein vivoconditions. How-ever, most often, enzyme–kinetic parameters are measured under assay con-ditions that yield the maximum activity of each enzyme. | Measuring enzyme activities under standardized in vivo-like conditions for systems biology Karen van Eunen1 2 Jildau Bouwman1 2 Pascale Daran-Lapujade2 3 Jarne Postmus4 Andre B. Canelas2 3 Femke I. C. Mensonides1 2 Rick Orij4 Isil Tuzun5 Joost van den Brink2 3 Gertien J. Smits4 Walter M. van Gulik2 3 Stanley Brul4 Joseph J. Heijnen2 3 Johannes H. de Winde2 3 M. J. Teixeira de Mattos5 Carsten Kettner6 Jens Nielsen7 Hans V. Westerhoff1 2 8 and Barbara M. Bakker1 2 9 1 Department of Molecular Cell Physiology Vrije Universiteit Amsterdam The Netherlands 2 Kluyver Centre for Genomics of Industrial Fermentation Delft The Netherlands 3 Department of Biotechnology Delft University of Technology The Netherlands 4 Department of Molecular Biology and Microbial Food Safety Swammerdam Institute for Life Sciences University of Amsterdam The Netherlands 5 Department of Molecular Micriobial Physiology Swammerdam Institute for Life Sciences University of Amsterdam The Netherlands 6 Beilstein-Institut zur Forderung der Chemischen Wissenschaften Frankfurt Main Germany 7 Department of Chemical and Biological Engineering Chalmers University of Technology Gothenburg Sweden 8 Manchester Centre for Integrative Systems Biology Manchester Interdisciplinary BioCentre The University of Manchester UK 9 Department of Paediatrics Centre for Liver Digestive and Metabolic Diseases University Medical Centre Groningen University of Groningen The Netherlands Keywords glycolysis in vivo enzyme kinetics modelling Saccharomyces cerevisiae standardization Correspondence B. M. Bakker Department of Paediatrics Centre for Liver Digestive and Metabolic Diseases University MedicalCentre Groningen University of Groningen Hanzeplein 1 NL-9713 GZ Groningen The Netherlands Fax 31 50 361 1746 Tel 31 50 361 1542 E-mail Note As a team and independently the authors are actively engaged in ongoing efforts of the internationalscientific community to define standards for yeast and other organisms

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