tailieunhanh - Báo cáo khoa học: Erythrochelin – a hydroxamate-type siderophore predicted from the genome of Saccharopolyspora erythraea

The class of nonribosomally assembled siderophores encompasses a multi-tude of structurally diverse natural products. The genome of the erythro-mycin-producing strainSaccharopolyspora erythraeacontains 25 secondary metabolite gene clusters that are mostly considered to be orphan, including two that are responsible for siderophore assembly. | Erythrochelin - a hydroxamate-type siderophore predicted from the genome of Saccharopolyspora erythraea Lars Robbel Thomas A. Knappe Uwe Linne Xiulan Xie and Mohamed A. Marahiel Department of Chemistry Philipps-University Marburg Germany Keywords genome mining nonribosomalpeptide synthetase radiolabeling secondary metabolites siderophore Correspondence M. A. Marahiel Department of Chemistry Philipps-University Marburg D-35043 Marburg Germany Fax 49 0 6421 282 2191 Tel 49 0 6421 282 5722 E-mail marahiel@ Received 4 October 2009 revised 10 November 2009 accepted 23 November 2009 doi The class of nonribosomally assembled siderophores encompasses a multitude of structurally diverse natural products. The genome of the erythro-mycin-producing strain Saccharopolyspora erythraea contains 25 secondary metabolite gene clusters that are mostly considered to be orphan including two that are responsible for siderophore assembly. In the present study we report the isolation and structural elucidation of the hydroxamate-type tetrapeptide siderophore erythrochelin the first nonribosomal peptide synthetase-derived natural product of S. erythraea. In an attempt to substitute the traditional activity assay-guided isolation of novel secondary metabolites we have employed a dedicated radio-LC-MS methodology to identify nonribosomal peptides of cryptic gene clusters in the industrially relevant strain. This methodology was based on transcriptome data and adenylation domain specificity prediction and resulted in the detection of a radiolabeled ornithine-inheriting hydroxamate-type siderophore. The improvement of siderophore production enabled the elucidation of the overall structure via NMR and MSn analysis and hydrolysate-derivatization for the determination of the amino acid configuration. The sequence of the tetrapeptide siderophore erythrochelin was determined to be D-a-N-acetyl-S-N-acetyl-S-N-hydroxyornithine-D-serine-cyclo .

• Báo cáo khoa học
• Scientific reports
• scientific research
• biochemistry
• Hydrogenobacter
• enzymatic
• biological activities
• Seagrass species
• Positive biological activities
• Coastal area of Vietnam
• Vietnam Sea
• Summary of Chemistry Doctoral Thesis
• Chemistry Doctoral Thesis
• Biological activities of callicarpa candicans
• Biological activities of callicarpa macrophylla
• Chemistry of natural compounds
• Summary of the Doctoral thesis
• Study on chemical constituents
• Biological activities of litsea glutinosa
• Lepisanthes rubiginosa
• Potential biological activities
• Biological activity
• Medicinal plants
• Biological screening
• Antitumor activities
• Mentha pulegium L
• Summary of biological doctoral thesis
• Research on mangrove vegetation
• Bological activities
• Xuan Thuy National Park
• Propose sustainable usage
• Typical mangrove vegetation communities
• Anti cancer
• Anti inflammatory
• Mallotus species
• Phytochemical and pharmacological activities
• Gallic acid
• Agricultural science
• Biological chemical
• Antioxidant activities
• Biological and chemical changes
• Acetic acid productivity
• Fermented tea
• Report medicine
• traditional medicine
• breast cancer
• environmental medicine
• Medical Research
• chemical reaction
• Công nghệ hóa
• Chemical constituents
• Alpinia conchigera griff
• Thành phần hóa học
• Hoạt tính sinh học
• Sedum acre
• Antiproliferative activity
• Goldmoss stonecrop
• Fetal bovine serum
• Vietnam Journal of Science and Technology
• Chemical constituents and biological activities
• Alpinia kwangsiensis collected in Vietnam
• Methanol extract of Alpinia kwangsiensis roots
• Staphylococcus aureus and Bacillus subtilis
• Tạp chí Phát triển Khoa học và Công nghệ
• Screening for some biological activities of Cultured cordyceps neovolkiana
• Cultured cordyceps neovolkiana
• C
• neovolkiana extracts
• Chemical constituents of Tacca vietnmensis and Tacca chantrierispecies
• Biological activities of Tacca vietnmensis
• Tacca chantrierispecies growing in Vietnam
• Find out bioactive compoundse
• Structural characteristics of fucoidans extracted
• Biological activities of fucoidans extracted
• Selected vietnamese brown seaweeds
• Several brown algae
• Antibacterial activity
• Anticancer activity
• Textile dyeing
• Natural colorant production
• BMC Chemistry
• Oxazole derivatives
• Heterocyclic systems
• Therapeutic potentials
• Anticancer activities
• Thiourea derivatives
• Exhibited biological activities
• Benzenesulfonamide derivatives
• Amberlyst A 15
• Various synthetic methods
• Indole and imidazole rings
• Synthetic approaches and biological activities
• Substituted indolylimidazole derivatives
• Oil properties DEH enzyme maize proline
• Influence of exogenous compounds
• Exogenous compounds
• Maize crop residue
• Salt affected soil
• Natural science
• Pseuderanthemum palatiferum
• Tyrosinase inhibitory activity
• Phytochemical constituents
• n hexane fractions
• Luận án Tiến sĩ
• Summary of Doctoral thesis in Chemistry
• Chemical compositions
• Paramignya trimera
• Organic chemistry
• Luận án Tiến sĩ Hóa học
• Antidesma species growing
• Fokienia hodginsii
• Dacrycarpus imbricatus