tailieunhanh - Báo cáo khoa học: Enhanced sensitivity to hydrogen peroxide-induced apoptosis in Evi1 transformed Rat1 fibroblasts due to repression of carbonic anhydrase III

EVI1 is a nuclear zinc finger protein essential to normal development, which participates in acute myeloid leukaemia progression and transforms Rat1 fibroblasts. In this study we show that enforced expression of Evi1 in Rat1 fibroblasts protects from paclitaxel-induced apoptosis, consistent with previously published studies. | ỊFEBS Journal Enhanced sensitivity to hydrogen peroxide-induced apoptosis in Evil transformed Rati fibroblasts due to repression of carbonic anhydrase III P. Roy1 E. Reavey1 M. Rayne1 S. Roy1 M. Abed El Baky1 Y. Ishii2 and C. Bartholomew1 1 Department of Biological BiomedicalSciences Glasgow Caledonian University City Campus Glasgow UK 2 Graduate Schoolof PharmaceuticalSciences Kyushu University Fukuoka Japan Keywords apoptosis carbonic anhydrase III Evi1 H2O2 Correspondence C. Bartholomew Department of Biological Biomedical Sciences Glasgow Caledonian University City Campus Cowcaddens Road Glasgow G4 OBA UK Fax 44 141 331 3208 Tel 44 141 331 3213 E-mail Received 18 August 2009 revised 9 November 2009 accepted 16 November 2009 doi EVI1 is a nuclear zinc finger protein essential to normal development which participates in acute myeloid leukaemia progression and transforms Rat1 fibroblasts. In this study we show that enforced expression of Evi1 in Rat1 fibroblasts protects from paclitaxel-induced apoptosis consistent with previously published studies. Surprisingly however these cells show increased sensitivity to hydrogen peroxide H2O2 -induced apoptosis demonstrated by elevated caspase 3 catalytic activity. This effect is caused by a reduction in carbonic anhydrase III caIII production. caIII transcripts are repressed by 92-97 by Evil expression accompanied by a similar reduction in caIII protein. Reporter assays with the rat caIII gene promoter show repressed activity demonstrating that Evi1 either directly or indirectly modulates transcription of this gene in Rat1 cells. Targeted knockdown of caIII alone with Dicer-substrate short inhibitory RNAs also increases the sensitivity of Rat1 fibroblasts to H2O2 which occurs in the absence of any other changes mediated by Evi1 expression. Enforced expression of caIII in Evil-expressing Ratl cells reverts the phenotype restoring H2O2 resistance. Together these data

• Báo cáo khoa học
• Scientific reports
• scientific research
• biochemistry
• carbonic anhydrase III
• fibroblasts
• chemical reaction
• Elements of Chemical Reaction Engineering
• Chemical Reaction Engineering
• Hóa học và ứng dụng
• Kỹ thuật hóa học
• Phản ứng hóa học
• Kỹ thuật phản ứng hóa học
• The chemical vapor deposition condition
• The electrochemically catalytic efficiency
• Hydrogen evolution reaction in MoS2 nanoparticles
• Hydrogen evolution reaction
• Metal organic chemical vapor deposition
• Reaction Kinetics
• Heterogeneous Catalysis
• Elementary Reactions
• Microkinetic Analysis
• Nonisothermal Reactors
• Catalytic Reactions
• Quantum chemical modeling mechanism
• Semiempirical method RM1
• Quantum chemical study
• Half chair shape
• Diels Alder reaction
• Chemical reaction engirneering
• Hoá phân tích
• Giải pháp phản ứng hóa học
• Radiation on MHD casson fluid flow
• Chemical reaction effects on MHD casson fluid flow
• Porous medium with suction injection
• The occurrence of suction/injection
• The central PDEs
• Radiation of MHD visco elastic fluid flow over
• Chemical reaction on heat of MHD visco elastic
• Mass transfer of MHD visco elastic fluid flow
• Exponentially stretching sheet through porous medium
• Radiation on the boundary layer MHD casson fluid
• Chemical reaction effects on the boundary layer MHD casson fluid
• Vertical plate embedded in the porous medium
• Casson fluid flow free convection boundary layer fl
• Extreme learning machine
• Artificial chemical reaction optimization algorithm
• Single hidden layer feed forward neural network
• Learning algorithm
• Chemical reaction kinetic
• Adsorption kinetics
• Pseudo first order model
• Pseudo second order model
• General kinetic model
• Dried Probiotics
• Industrial Processes
• Membrane Separation
• Wastewater
• Maillard Reaction
• Pros and Cons
• Chemical kinetics
• Reactant concentration
• Reaction rates and stoichiometry
• Action of catalysts
• Surface area
• Chemical kinetics
• Introduction to general
• Chemical bonds
• Chemical reactions
• Reaction rates
• Chemical equilibrium
• Nuclear chemistry
• Nuclear engineering and technology
• Correlation between rare earth elements
• The chemical interactions of HT9 cladding
• FueleCladding Chemical Interaction
• Higher reaction thickness
• Summary of Chemical PhD Thesis
• Theoretical and Physical Chemistry
• CO hydrogenation reaction
• Ni Cu bimetallic catalyst system
• Density functional method
• Chemical method
• Lecture General Chemistry 1
• Bài giảng Hóa học đại cương 1
• Balance chemical equations
• Calculate the percent yield of a reaction
• Chemical formulas
• Chemical equations
• organic chemistry
• hóa hữu cơ
• công nghệ hóa học
• Chemical Engineering
• common reaction mechanisms
• Chromatography in the petroleum industry
• Report medicine
• traditional medicine
• oxidation
• tumor cells
• BMC Bioinformatics
• Flux balance analysis
• Linear programming
• Design of metabolic network
• Constraint based model
• Growth rate
• Production rate
• Smaller reaction network
• Luận án Tiến sĩ
• Physical chemistry
• Fabbricated electrocatalysts
• Oxygen evolution reaction
• Proton exchange membrane
• breast cancer
• medicine
• cytotoxicity
• Atomic nucleus
• electrons
• Chemical compounds
• Electrochemistry