tailieunhanh - Báo cáo khoa học: Restriction point control of the mammalian cell cycle via the cyclin E/Cdk2:p27 complex

An increasing amount of expressed sequence tag (EST) and genomic data, predominantly for the cnidarians Acropora, Hydraand Nematostella, reveals that cnidarians have a high genomic complexity, despite being one of the morphologically simplest multicellular animals. | ỊFEBS Journal Restriction point control of the mammalian cell cycle via the cyclin E Cdk2 p27 complex Riaan Conradie1 Frank J. Bruggeman2 Andrea Ciliberto3 Attila Csikasz-Nagy4 Bela Novak5 Hans V. Westerhoff2 6 and Jacky L. Snoep1 2 6 1 Triple J Group for Molecular CellPhysiology Department of Biochemistry Stellenbosch University Matieland South Africa 2 Molecular CellPhysiology Netherlands Institute for Systems Biology Vrije Universiteit Amsterdam The Netherlands 3 FIRC Institute of Molecular Oncology Foundation Milan Italy 4 University of Trento Centre for Computationaland Systems Biology Povo Trento Italy 5 Oxford Centre for Integrative Systems Biology University of Oxford UK 6 Manchester Centre for Integrative Systems Biology Manchester Interdisciplinary Biocentre The University of Manchester UK Keywords cell cycle kinetic modeling metabolic control analysis restriction point systems biology Correspondence J. L. Snoep Triple J Group for Molecular CellPhysiology Department of Biochemistry Stellenbosch University Private Bag X1 Matieland 7602 South Africa Fax 272 1808 5863 Tel 272 1808 5844 E-mail jls@ Note The mathematical model described here has been submitted to the JWS Online Cellular Systems Modelling Database and can be accessed at http database conradie of charge Received 14 May 2009 revised 21 October 2009 accepted 30 October 2009 doi Numerous top-down kinetic models have been constructed to describe the cell cycle. These models have typically been constructed validated and analyzed using model species molecular intermediates and proteins and phenotypic observations and therefore do not focus on the individual model processes reaction steps . We have developed a method to a quantify the importance of each of the reaction steps in a kinetic model for the positioning of a switch point . the restriction point RP b relate this control of reaction steps to their effects on .