tailieunhanh - báo cáo khoa học: "Ganoderma lucidum polysaccharides can induce human monocytic leukemia cells into dendritic cells with immuno-stimulatory function"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài:Ganoderma lucidum polysaccharides can induce human monocytic leukemia cells into dendritic cells with immuno-stimulatory function | BioMed Central Journal of Hematology Oncology Open Access Ganoderma lucidum polysaccharides can induce human monocytic leukemia cells into dendritic cells with immuno-stimulatory function Wing Keung Chan Christopher Ching Hang Cheung Helen Ka Wai Law Yu Lung Lau and Godfrey Chi Fung Chan Address Department of Paediatrics Adolescent Medicine Li Ka Shing Faculty of Medicine The University of Hong Kong Hong Kong PR China Email Wing Keung Chan - wingkc@ Christopher Ching Hang Cheung - Helen Ka Wai Law - hkwlaw@ Yu Lung Lau - lauylung@ Godfrey Chi Fung Chan - gcfchan@ Corresponding author Published 21 July 2008 Received 13 May 2008 Journal of Hematology Oncology 2008 1 9 doi 1756-8722-1 -9 Accepted 21 July 2008 This article is available from http content 1 1 9 2008 Chan et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract_ Background Previous studies demonstrated Ganoderma lucidum polysaccharides GL-PS a form of bioactive P-glucan can stimulate the maturation of monocyte-derived dendritic cells DC . The question of how leukemic cells especially in monocytic lineage respond to GL-PS stimuli remains unclear. Results In this study we used in vitro culture model with leukemic monocytic cell-lines THP-1 and U937 as monocytic effectors cells for proliferation responses and DCs induction. We treated the THP-1 and U937 cells with purified gL-PS 100 pg mL or GL-PS with GM-CSF IL-4. GL-PS alone induced proliferative response on both THP-1 and U937 cells but only THP-1 transformed into typical DC morphology when stimulated with GL-PS plus GM-CSF IL-4. The .