tailieunhanh - báo cáo khoa học: "Mitochondrial and endoplasmic reticulum stress pathways cooperate in zearalenone-induced apoptosis of human leukemic cells"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài:Mitochondrial and endoplasmic reticulum stress pathways cooperate in zearalenone-induced apoptosis of human leukemic cells | Banjerdpongchai et al. Journal of Hematology Oncology 2010 3 50 http content 3 1 50 JOURNAL OF HEMATOLOGY ONCOLOGY RESEARCH Open Access Mitochondrial and endoplasmic reticulum stress pathways cooperate in zearalenone-induced apoptosis of human leukemic cells 1 1 1 2 Ratana Banjerdpongchai Prachya Kongtawelert Orawan Khantamat Chantragan Srisomsap Daranee Chokchaichamnankit2 Pantipa Subhasitanont2 Jisnuson Svasti2 3 Abstract Background Zearalenone ZEA is a phytoestrogen from Fusarium species. The aims of the study was to identify mode of human leukemic cell death induced by ZEA and the mechanisms involved. Methods Cell cytotoxicity of ZEA on human leukemic HL-60 U937 and peripheral blood mononuclear cells PBMCs was performed by using 3- 4 5-dimethyl -2 5-diphenyl tetrazolium bromide MTT assay. Reactive oxygen species production cell cycle analysis and mitochondrial transmembrane potential reduction was determined by employing 2 7 -dichlorofluorescein diacetate propidium iodide and 3 3 -dihexyloxacarbocyanine iodide and flow cytometry respectively. Caspase-3 and -8 activities were detected by using fluorogenic Asp-Glu-Val-Asp-7-amino-4-methylcoumarin DEVD-AMC and Ile-Glu-Thr-Asp-7-amino-4-methylcoumarin IETD-AMC substrates respectively. Protein expression of cytochrome c Bax Bcl-2 and Bcl-xL was performed by Western blot. The expression of proteins was assessed by two-dimensional polyacrylamide gel-electrophoresis PAGE coupled with LC-MS2 analysis and realtime reverse transcription polymerase chain reaction RT-PCR approach. Results ZEA was cytotoxic to U937 HL-60 PBMCs and caused subdiploid peaks and G1 arrest in both cell lines. Apoptosis of human leukemic HL-60 and U937 cell apoptosis induced by ZEA was via an activation of mitochondrial release of cytochrome c through mitochondrial transmembrane potential reduction activation of caspase-3 and -8 production of reactive oxygen species and induction of endoplasmic reticulum stress. Bax was up .