tailieunhanh - báo cáo khoa học: "Phenotype and functional evaluation of ex vivo generated antigen-specific immune effector cells with potential for therapeutic applications"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài:Phenotype and functional evaluation of ex vivo generated antigen-specific immune effector cells with potential for therapeutic applications | BioMed Central Journal of Hematology Oncology Research Phenotype and functional evaluation of ex vivo generated antigen-specific immune effector cells with potential for therapeutic applications Shuhong Han1 Yuju Huang2 Yin Liang2 Yuchin Ho2 Yichen Wang2 and Lung-Ji Chang 1 Open Access Address Department of Molecular Genetics and Microbiology College of Medicine University of Florida Gainesville FL 32610-0266 and 2Vectorite Biomedica Inc. Taipei Taiwan Republic of China Email Shuhong Han - hansh72@ Yuju Huang - Yin Liang - Yuchin Ho - Malineho@ Yichen Wang - Lung-Ji Chang - lchang@ Corresponding author Published 6 August 2009 Received 30 June 2009 Journal of Hematology Oncology 2009 2 34 doi 1756-8722-2-34 Accepted 6 August 2009 This article is available from http content 2 1 34 2009 Han et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Ex vivo activation and expansion of lymphocytes for adoptive cell therapy has demonstrated great success. To improve safety and therapeutic efficacy increased antigen specificity and reduced nonspecific response of the ex vivo generated immune cells are necessary. Here using a complete protein-spanning pool of pentadecapeptides of the latent membrane protein 2A LMP2A of Epstein-Barr virus EBV a weak viral antigen which is associated with EBV lymphoproliferative diseases we investigated the phenotype and function of immune effector cells generated based on IFN-y or CD137 activation marker selection and dendritic cell DC activation. These ex vivo prepared immune cells exhibited a donor- and antigen-dependent T cell response the IFN-y-selected immune cells