tailieunhanh - báo cáo khoa học: "Induction of endogenous γ-globin gene expression with decoy oligonucleotide targeting Oct-1 transcription factor consensus sequence"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Induction of endogenous γ-globin gene expression with decoy oligonucleotide targeting Oct-1 transcription factor consensus sequence | BioMed Central Journal of Hematology Oncology Research Induction of endogenous Y-globin gene expression with decoy oligonucleotide targeting Oct-1 transcription factor consensus sequence Xiaoxin S Xu Xin Hong and Gan Wang Address Institute of Environmental Health Sciences Wayne State University 2727 Second Avenue Detroit MI 48201 USA Email Xiaoxin S Xu - Xin Hong - nsxin@ Gan Wang - Corresponding authors Open Access Published 27 March 2009 Received 18 December 2008 Journal of Hematology Oncology 2009 2 15 doi 1756-8722-2-15 Accepted 27 March 2009 This article is available from http content 2 1 15 2009 Xu et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Human P-globin disorders are relatively common genetic diseases cause by mutations in the P-globin gene. Increasing the expression of the y-globin gene has great benefits in reducing complications associated with these diseases. The Oct-1 transcription factor is involved in the transcriptional regulation of the Y-globin gene. The human y-globin genes both Ay and Gy-globin genes carry three Oct-1 transcription factor consensus sequences within their promoter regions. We have studied the possibility of inducing y-globin gene expression using decoy oligonucleotides that target the Oct-1 transcription factor consensus sequence. A double-stranded 22 bp decoy oligonucleotide containing the Oct-1 consensus sequence was synthesized. The results obtained from our in vitro binding assay revealed a strong competitive binding of the decoy oligonucleotide for the Oct-1 transcription factor. When K562 human erythroleukemia cells were treated with the Oct-1 decoy oligonucleotide significant increases in the