tailieunhanh - báo cáo khoa học: "SET-NUP214 fusion in acute myeloid leukemia- and T-cell acute lymphoblastic leukemia-derived cell lines"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: SET-NUP214 fusion in acute myeloid leukemia- and T-cell acute lymphoblastic leukemia-derived cell lines | Journal of Hematology Oncology BioMed Central Open Access Short report SET-NUP214 fusion in acute myeloid leukemia- and T-cell acute lymphoblastic leukemia-derived cell lines Hilmar Quentmeier Bjorn Schneider Sonja Rohrs Julia Romani Margarete Zaborski Roderick AF MacLeod and Hans G Drexler Address DSMZ-German Collection of Microorganisms and Cell Cultures Braunschweig Germany Email Hilmar Quentmeier - hqu@ Bjorn Schneider - Sonja Rohrs - Julia Romani - jrh@ Margarete Zaborski - mza@ Roderick AF MacLeod - rml@ Hans G Drexler - hdr@ Corresponding author Published 23 January 2009 Received 25 November 2008 Journal of Hematology Oncology 2009 2 3 doi 1756-8722-2-3 Accepted 23 January 2009 This article is available from http content 2 1 3 2009 Quentmeier et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background SET-NUP214 fusion resulting from a recurrent cryptic deletion del 9 has recently been described in T-cell acute lymphoblastic leukemia T-ALL and in one case of acute myeloid leukemia AML . The fusion protein appears to promote elevated expression of HOXA cluster genes in T-ALL and may contribute to the pathogenesis of the disease. We screened a panel of ALL and AML cell lines for SET-NUP214 expression to find model systems that might help to elucidate the cellular function of this fusion gene. Results Of 141 human leukemia lymphoma cell lines tested only the T-ALL cell line LOUCY and the AML cell line MEGAL expressed the SET TAF-IP -NUP214 fusion gene transcript. RT-PCR analysis specifically recognizing the alternative first exons of the two TAF-I isoforms revealed that the cell lines .