tailieunhanh - Báo cáo y học: "Significance of differential expression of thymidylate synthase in normal and primary tumor tissues from patients with colorectal cancer"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Significance of differential expression of thymidylate synthase in normal and primary tumor tissues from patients with colorectal cancer. | Liu et al. Journal of Hematology Oncology 2011 4 33 http content 4 1 33 JOURNAL OF HEMATOLOGY ONCOLOGY LETTER TO THE EDITOR Open Access Significance of differential expression of thymidylate synthase in normal and primary tumor tissues from patients with colorectal cancer 1 t w 2t 1t 12 3 1 Yanyan Liu Qingxin Xia Yanzhao Jia Hongqiang Guo Bing Wei Yawei Hua and Shujun Yang Abstract The role of thymidylate synthase TS is essential as a key rate-limiting enzyme in DNA synthesis. It is the primary target of fluorouracil and its derivates in colorectal cancer. In this study TS mRNA expression was examined in primary tumor and normal tissues from 76 patients with high- risk stage II III colorectal cancer by laser capture microdissection and polymerase chain reaction. Thirty patients were found to have higher TS expression in primary tumors with earlier stage P lower histological grades P and high frequency microsatellite instability P . Multivariate analysis showed that microsatellite instability histological grade and number of lymph nodes examined are independent prognostic markers. To the editor Adjuvant chemotherapy can prolong survival of patients with high-risk stage II III colorectal cancer CRC after curative surgical resection 1-3 . Thymidylate synthase TS plays a major role as a key rate-limiting enzyme in DNA synthesis and serves as the primary target of fluorouracil and its derivates in CRC. It appears to have predictive and prognostic values 4 5 . Although the differences in TS expression among primary tumors lymph nodes and other metastases have been reported 6 the pattern of TS expression in normal and tumor tissues has not been well characterized. In this study Laser capture microdissection LCM combined with polymerase chain reaction was used to examine TS gene expression in 76 patients with high risk II III stage CRC Table 1 . Microsatellite instability MSI a panel of five loci recommended by National Cancer .