tailieunhanh - Báo cáo y học: "Accelerated cellular senescence in degenerate intervertebral discs: a possible role in the pathogenesis of intervertebral disc degeneration"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Accelerated cellular senescence in degenerate intervertebral discs: a possible role in the pathogenesis of intervertebral disc degeneration. | Available online http content 9 3 R45 Research article Accelerated cellular senescence in degenerate intervertebral discs a possible role in the pathogenesis of intervertebral disc degeneration Christine Lyn Le Maitre Anthony John Freemont and Judith Alison Hoyland Tissue Injury and Repair Group School of Medicine Stopford Building The University of Manchester Oxford Road Manchester UK M13 9PT Corresponding author Judith Alison Hoyland Received 13 Mar 2007 Revisions requested 16 Apr 2007 Revisions received 26 Apr 2007 Accepted 11 May 2007 Published 11 May 2007 Arthritis Research Therapy 2007 9 R45 doi ar2198 This article is online at http content 9 3 R45 2007 Le Maitre et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Open Access Abstract Current evidence implicates intervertebral disc degeneration as a major cause of low back pain although its pathogenesis is poorly understood. Numerous characteristic features of disc degeneration mimic those seen during ageing but appear to occur at an accelerated rate. We hypothesised that this is due to accelerated cellular senescence which causes fundamental changes in the ability of disc cells to maintain the intervertebral disc IVD matrix thus leading to IVD degeneration. Cells isolated from non-degenerate and degenerate human tissue were assessed for mean telomere length senescence-associated p-galactosidase SA-p-gal and replicative potential. Expression of P16INK4A increased in cellular senescence was also investigated in IVD tissue by means of immunohistochemistry. RNA from tissue and cultured cells was used for real-time polymerase chain reaction analysis for matrix metalloproteinase-13 .