tailieunhanh - Báo cáo y học: "Inhibition of hyaluronan export reduces collagen degradation in interleukin-1 treated cartilage"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Inhibition of hyaluronan export reduces collagen degradation in interleukin-1 treated cartilage. | Available online http content 10 1 R8 Research article Inhibition of hyaluronan export reduces collagen degradation in interleukin-1 treated cartilage Barthold Deiters and Peter Prehm Open Access Muenster University Hospital Institute of Physiological Chemistry and Pathobiochemistry Waldeyerstr. 15 D-48129 Munster Germany Corresponding author Peter Prehm prehm@ Received 31 Jul 2007 Revisions requested 29 Aug 2007 Revisions received 25 Oct 2007 Accepted 18 Jan 2008 Published 18 Jan 2008 Arthritis Research Therapy 2008 10 R8 doi 1 ar2357 This article is online at http content 10 1 R8 2008 Deiters and Prehm licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Osteoarthrosis is characterized by cartilage erosion proteolysis of aggrecan and collagen and disturbed rates of synthesis of aggrecan and hyaluronan by chondrocytes with hyaluronan over-production being an early reaction. We considered that inhibition of hyaluronan export might prevent subsequent proteoglycan loss and collagen degradation. Methods To test this hypothesis we studied a tissue culture model using bovine cartilages explants activated with IL-1a to induce osteoarthritic reactions using the phosphodiesterase-5 inhibitors tadalafil zaprinast and vardenafil. Results These drugs inhibited hyaluronan export but they did not inhibit hyaluronan synthase activity. Simultaneously they inhibited proteoglycan loss and collagen degradation but not their synthesis. They also reduced the release of gelatinases into the culture media and diffusion of the indicator protein horseradish peroxidase through the cartilage explants. The mechanism of action of these compounds may be through .