tailieunhanh - Báo cáo y học: "Human infrapatellar fat pad-derived stem cells express the pericyte marker 3G5 and show enhanced chondrogenesis after expansion in fibroblast growth factor-2"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Human infrapatellar fat pad-derived stem cells express the pericyte marker 3G5 and show enhanced chondrogenesis after expansion in fibroblast growth factor-2. | Available online http content 10 4 R74 Research article Human infrapatellar fat pad-derived stem cells express the pericyte marker 3G5 and show enhanced chondrogenesis after expansion in fibroblast growth factor-2 Wasim S Khan Simon R Tew Adetola B Adesida and Timothy E Hardingham United Kingdom Centre for Tissue Engineering at the Wellcome Trust Centre for Cell Matrix Research Faculty of Life Sciences Michael Smith Building University of Manchester Oxford Road Manchester M13 9PT UK Corresponding author Wasim S Khan wasimkhan@ Received 5 Jul 2007 Revisions requested 6 Sep 2007 Revisions received 18 Jun 2008 Accepted 3 Jul 2008 Published 3 Jul 2008 Arthritis Research Therapy 2008 10 R74 doi ar2448 This article is online at http content 10 4 R74 2008 Khan et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Open Access Abstract Introduction Infrapatellar fat pad IPFP is a possible source of stem cells for the repair of articular cartilage defects. In this study adherent proliferative cells were isolated from digests of IPFP tissue. The effects of the expansion of these cells in fibroblast growth factor-2 FGF-2 were tested on their proliferation characterisation and chondrogenic potential. Methods IPFP tissue was obtained from six patients undergoing total knee replacement and sections were stained with 3G5 alpha smooth muscle actin and von Willebrand factor to identify different cell types in the vasculature. Cells were isolated from IPFP and both mixed populations and clonal lines derived from them were characterised for cell surface epitopes including 3G5. Cells were expanded with and without FGF-2 and were tested for chondrogenic differentiation in cell