tailieunhanh - Báo cáo y học: " Importance of spliceosomal RNP1 motif for intermolecular T-B cell spreading and tolerance restoration in lupus"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Importance of spliceosomal RNP1 motif for intermolecular T-B cell spreading and tolerance restoration in lupus. | Available online http content 9 5 R111 Research article Importance of spliceosomal RNP1 motif for intermolecular T-B cell spreading and tolerance restoration in lupus Fanny Monneaux Véronique Parietti Jean-Paul Briand and Sylviane Muller Centre National de la Recherche Scientifique UPR9021 Institut de Biologie Moléculaire et Cellulaire 15 rue René Descartes 67000 Strasbourg France Corresponding author Sylviane Muller Received 5 Jul 2007 Revisions requested 31 Jul 2007 Revisions received 7 Aug 2007 Accepted 26 Oct 2007 Published 26 Oct 2007 Arthritis Research Therapy 2007 9 R111 doi ar231 7 This article is online at http content 9 5 R111 2007 Monneaux et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Open Access Abstract We previously demonstrated the importance of the RNP1 motifbearing region 131-151 of the U1-70K spliceosomal protein in the intramolecular T-B spreading that occurs in MRL lpr lupus mice. Here we analyze the involvement of RNP1 motif in the development and prevention of naturally-occurring intermolecular T-B cell diversification. We found that MRL lpr peripheral blood lymphocytes proliferated in response to peptides containing or corresponding exactly to the RNP1 motif of spliceosomal U1-70K U1-A and hnRNP-A2 proteins. We also demonstrated that rabbit antibodies to peptide 131-151 cross-reacted with U1-70K U1-A and hnRNP-A2 RNP1-peptides. These antibodies recognized the U1-70K and U1-A proteins and also U1-C and SmD1 proteins which are devoid of RNP1 motif. Repeated administration of phosphorylated peptide P140 into MRL lpr mice abolished T-cell response to several peptides from the U1-70K U1-A and SmD1 proteins without .