tailieunhanh - Báo cáo khoa học: " Counting colonies of clonogenic assays by using densitometric software"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Radiation Oncology cung cấp cho các bạn kiến thức về ngành y đề tài: " Counting colonies of clonogenic assays by using densitometric software. | Radiation Oncology BioMed Central Open Access Methodology Counting colonies of clonogenic assays by using densitometric software Maximilian Niyazi 1 Ismat Niyazi2 and Claus Belka1 Address 1CCC Tubingen Department of Radiation Oncology Hoppe-Seyler-Str. 3 72076 Tubingen Germany and 2Bureau for Technique and Documentation Paracelsusstr. 21 70599 Stuttgart Germany Email Maximilian Niyazi - maxi@ Ismat Niyazi - ismat@ Claus Belka - Corresponding author Published 09 January 2007 Received 13 December 2006 Radiation Oncology 2007 2 4 doi 1748-7I7X-2-4 Accepted 09 January 2007 This article is available from http content 2 1 4 2007 Niyazi et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract_ Clonogenic assays are a useful tool to test whether a given cancer therapy can reduce the clonogenic survival of tumour cells. A colony is defined as a cluster of at least 50 cells which can often only be determined microscopically. The process of counting colonies is very extensive work and so we developed software that is able to count the colonies automatically from scanned flasks. This software is made freely available by us with a detailed description how to use and install the necessary features. Background Everyone who has already counted colonies from a clonogenic assay knows that this is hard work which takes a lot of time. Many groups have thought about an improvement of the counting system 1-8 and the cited publications certainly won t cover all attempts but no method has achieved a widespread use at all. One may ask what the reasons are. Two major reasons are certainly the .