tailieunhanh - Báo cáo y học: " High abundance synovial fluid proteome: distinct profiles in health and osteoarthritis"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: High abundance synovial fluid proteome: distinct profiles in health and osteoarthritis. | Available online http content 9 2 R36 Research article High abundance synovial fluid proteome distinct profiles in health and osteoarthritis Reuben Gobezie1 Alvin Kho1 Bryan Krastins1 David A Sarracino1 Thomas S Thornhill1 Michael Chase1 Peter J Millett1 and David M Lee1 2 Open Access 1The Case Center for Proteomics Case Western Reserve University School of Medicine Euclid Avenue Cleveland Ohio 44106 USA department of Rheumatology Brigham and Women s Hospital Harvard Medical School Boston MA 02115 USA Corresponding author Reuben Gobezie Received 16 Jan 2007 Revisions requested 8 Feb 2007 Revisions received 6 Mar 2007 Accepted 2 Apr 2007 Published 2 Apr 2007 Arthritis Research Therapy 2007 9 R36 doi ar2172 This article is online at http content 9 2 R36 2007 Gobezie et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract The development of increasingly high-throughput and sensitive mass spectroscopy-based proteomic techniques provides new opportunities to examine the physiology and pathophysiology of many biologic fluids and tissues. The purpose of this study was to determine protein expression profiles of high-abundance synovial fluid SF proteins in health and in the prevalent joint disease osteoarthritis OA . A cross-sectional study of 62 patients with early OA n 21 patients with late OA n 21 and control individuals n 20 was conducted. SF proteins were separated by using one-dimensional PAGE and the in-gel digested proteins were analyzed by electrospray ionization tandem mass spectrometry. A total of 362 spots were examined and 135 high-abundance SF proteins were identified as being expressed across all three study cohorts. A total of 135