tailieunhanh - Báo cáo khoa học: " Prediction of clinical toxicity in locally advanced head and neck cancer patients by radio-induced apoptosis in peripheral blood lymphocytes (PBLs)"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Radiation Oncology cung cấp cho các bạn kiến thức về ngành y đề tài: " Prediction of clinical toxicity in locally advanced head and neck cancer patients by radio-induced apoptosis in peripheral blood lymphocytes (PBLs). | Bordón et al. Radiation Oncology 2010 5 4 http content 5 1 4 RADIATION ONCOLOGY SHORT REPORT Open Access Prediction of clinical toxicity in locally advanced head and neck cancer patients by radio-induced apoptosis in peripheral blood lymphocytes PBLs Elisa Bordón 1 Luis Alberto Henriquez-Hernandez1 2 Pedro C Lara1 3 Ana Ruiz3 Beatriz Pinar1 3 Carlos Rodríguez-Gallego1 4 Marta Lloret1 3 Abstract Head and neck cancer is treated mainly by surgery and radiotherapy. Normal tissue toxicity due to x-ray exposure is a limiting factor for treatment success. Many efforts have been employed to develop predictive tests applied to clinical practice. Determination of lymphocyte radio-sensitivity by radio-induced apoptosis arises as a possible method to predict tissue toxicity due to radiotherapy. The aim of the present study was to analyze radio-induced apoptosis of peripheral blood lymphocytes in head and neck cancer patients and to explore their role in predicting radiation induced toxicity. Seventy nine consecutive patients suffering from head and neck cancer diagnosed and treated in our institution were included in the study. Toxicity was evaluated using the Radiation Therapy Oncology Group scale. Peripheral blood lymphocytes were isolated and irradiated at 0 1 2 and 8 Gy during 24 hours. Apoptosis was measured by flow cytometry using annexin V propidium iodide. Lymphocytes were marked with CD45 APC-conjugated monoclonal antibody. Radiation-induced apoptosis increased in order to radiation dose and fitted to a semi logarithmic model defined by two constants a and p. a as the origin of the curve in the Y axis determining the percentage of spontaneous cell death and p as the slope of the curve determining the percentage of cell death induced at a determined radiation dose were obtained. p value was statistically associated to normal tissue toxicity in terms of severe xerostomia as higher levels of apoptosis were observed in patients with low toxicity p