tailieunhanh - Báo cáo khoa học: "The interaction between different types of activated RAW 264.7 cells and macrophage inflammatory protein-1 alpha"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Radiation Oncology cung cấp cho các bạn kiến thức về ngành y đề tài: The interaction between different types of activated RAW cells and macrophage inflammatory protein-1 alpha. | He et al. Radiation Oncology 2011 6 86 http content 6 1 86 RADIATION ONCOLOGY RESEARCH Open Access The interaction between different types of activated RAW cells and macrophage inflammatory protein-1 alpha S-Ị z 71 z 71 2 1 Zhongshi He Hui Zhang Chunxu Yang Yajuan Zhou Yong Zhou Guang Han Ling Xia Wen Ouyang1 Fuxiang Zhou1 Yunfeng Zhou1 and Conghua Xie1 Abstract Background Two major ways of macrophage MO activation can occur in radiation-induced pulmonary injury RPI classical and alternative MO activation which play important roles in the pathogenesis of RPI. MO can produce chemokine MO inflammatory protein-1a MIP-1a while MIP-1a can recruit MO. The difference in the chemotactic ability of MIP-1a toward distinct activated MO is unclear. We speculated that there has been important interaction of MIP-1a with different activated MO which might contribute to the pathogenesis of RPI. Methods Classically and alternatively activated MO were produced by stimulating murine MO cell line RAW cells with three different stimuli LPS IL-4 and IL-13 Then we used recombinant MIP-1a to attract two types of activated MO. In addition we measured the ability of two types of activated MO to produce MIP-1a at the protein or mRNA level. Results Chemotactic ability of recombinant MIP-1a toward IL-13-treated MO was the strongest was moderate for IL-4-treated MO and was weakest for LPS-stimulated MO p . The ability of LPS-stimulated MO to secrete MIP-1a was significantly stronger than that of IL-4-treated or IL-13-treated MO p . The ability of LPS-stimulated MO to express MIP-1a mRNA also was stronger than that of IL-4- or IL-13-stimulated MO p . Conclusions The chemotactic ability of MIP-1a toward alternatively activated MO M2 was significantly greater than that for classically activated MO M1 . Meanwhile both at the mRNA and protein level the capacity of M1 to produce MIP-1a is better than that of M2. Thus chemokine MIP-1a .

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