tailieunhanh - Báo cáo khoa học: "Cationized gelatin-HVJ envelope with sodium borocaptate improved the BNCT efficacy for liver tumors in vivo"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Radiation Oncology cung cấp cho các bạn kiến thức về ngành y đề tài: Cationized gelatin-HVJ envelope with sodium borocaptate improved the BNCT efficacy for liver tumors in vivo. | Fujii et al. Radiation Oncology 2011 6 8 http content 6 1 8 RADIATION ONCOLOGY RESEARCH Open Access Cationized gelatin-HVJ envelope with sodium borocaptate improved the BNCT efficacy for liver tumors in vivo 1 2 12 3 4 Hitoshi Fujii Akifumi Matsuyama Hiroshi Komoda Masao Sasai Minoru Suzuki Tomoyuki Asano Vi lirhỈrr t F lr il i1 j li-f-ri Ifir iri I4irih4 Cbnr 13 Cl IHikm Tojk ol O 5 Voici iT imi IGnord6 VmcHiki 1 Yuichiro Doki Mitsunori Kirihata Koji Ono Yasuhiko Tabata Yasufumi Kaneda Yoshiki Sawa Chun Man Lee1 2 7 Abstract Background Boron neutron capture therapy BNCT is a cell-selective radiation therapy that uses the alpha particles and lithium nuclei produced by the boron neutron capture reaction. BNCT is a relatively safe tool for treating multiple or diffuse malignant tumors with little injury to normal tissue. The success or failure of BNCT depends upon the 10B compound accumulation within tumor cells and the proximity of the tumor cells to the body surface. To extend the therapeutic use of BNCT from surface tumors to visceral tumors will require 10B compounds that accumulate strongly in tumor cells without significant accumulation in normal cells and an appropriate delivery method for deeper tissues. Hemagglutinating Virus of Japan Envelope HVJ-E is used as a vehicle for gene delivery because of its high ability to fuse with cells. However its strong hemagglutination activity makes HVJ-E unsuitable for systemic administration. In this study we developed a novel vector for 10B sodium borocaptate BSH delivery using HVJ-E and cationized gelatin for treating multiple liver tumors with BNCT without severe adverse events. Methods We developed cationized gelatin conjugate HVJ-E combined with BSH CG-HVJ-E-BSH and evaluated its characteristics toxicity affinity for tumor cells accumulation and retention in tumor cells boron-carrying capacity to multiple liver tumors in vivo and bio-distribution and effectiveness in BNCT therapy in a murine model