tailieunhanh - Báo cáo khoa học: "Effect of neoadjuvant chemoradiation and postoperative radiotherapy on expression of heat shock protein 70 (HSP70) in head and neck vessels"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Radiation Oncology cung cấp cho các bạn kiến thức về ngành y đề tài: Effect of neoadjuvant chemoradiation and postoperative radiotherapy on expression of heat shock protein 70 (HSP70) in head and neck vessels. | Tavassol et al. Radiation Oncology 2011 6 81 http content 6 1 81 RADIATION ONCOLOGY RESEARCH Open Access Effect of neoadjuvant chemoradiation and postoperative radiotherapy on expression of heat shock protein 70 HSP70 in head and neck vessels Frank Tavassol Horst Kokemuller Rudiger Zimmerer Nils-Claudius Gellrich and André Eckardt Abstract Background Preoperative radiotherapy and chemotherapy in patients with head and neck cancer result in changes to the vessels that are used to construct microsurgical anastomoses. The aim of the study was to investigate quantitative changes and HSP70 expression of irradiated neck recipient vessels and transplant vessels used for microsurgical anastomoses. Methods Of 20 patients included in this study five patients received neoadjuvant chemoradiation another five received conventional radiotherapy and 10 patients where treated without previous radiotherapy. During surgical procedure vessel specimens where obtained by the surgeon. Immunhistochemical staining of HSP70 was performed and quantitative measurement and evaluation of HSP70 was carried out. Results Conventional radiation and neoadjuvant chemoradiation revealed in a thickening of the intima layer of recipient vessels. A increased expression of HSP70 could be detected in the media layer of the recipient veins as well as in the transplant veins of patients treated with neoadjuvant chemoradiation. Radiation and chemoradiation decreased the HSP70 expression of the intima layer in recipient arteries. Conventional radiation led to a decrease of HSP70 expression in the media layer of recipient arteries. Conclusion Our results showed that anticancer drugs can lead to a thickening of the intima layer of transplant and recipient veins and also increase the HSP70 expression in the media layer of the recipient vessels. In contrast conventional radiation decreased the HSP70 expression in the intima layer of arteries and the media layer of recipient arteries and veins.