tailieunhanh - Báo cáo y học: "Effects of hyaluronan treatment on lipopolysaccharide-challenged fibroblast-like synovial cell"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Effects of hyaluronan treatment on lipopolysaccharide-challenged fibroblast-like synovial cells. | Available online http content 9 1 R1 Research article Effects of hyaluronan treatment on lipopolysaccharide-challenged fibroblast-like synovial cells Kelly S Santangelo1 Amanda L Johnson1 Amy S Ruppert2 and Alicia L Bertone1 Department of Clinical Sciences College of Veterinary Medicine The Ohio State University 1900 Coffey Road Columbus OH 43210 USA 2Center for Biostatistics The Ohio State University 320 West 10th Avenue Columbus OH 43210 USA Corresponding author Alicia L Bertone Received 3 Jun 2006 Revisions requested 20 Jul 2006 Revisions received 1 9 Dec 2006 Accepted 10 Jan 2007 Published 10 Jan 2007 Arthritis Research Therapy 2007 9 R1 doi ar21 04 This article is online at http content 9 1 R1 2007 Santangelo et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Open Access Abstract Numerous investigations have reported the efficacy of exogenous hyaluronan HA in modulating acute and chronic inflammation. The current study was performed to determine the in vitro effects of lower and higher molecular weight HA on lipopolysaccharide LPS -challenged fibroblast-like synovial cells. Normal synovial fibroblasts were cultured in triplicate to one of four groups group 1 unchallenged group 2 LPS-challenged 20 ng ml group 3 LPS-challenged following preteatment and sustained treatment with lower molecular weight HA and group 4 LPS-challenged following pretreatment and sustained treatment with higher molecular weight HA. The response to LPS challenge and the influence of HA were compared among the four groups using cellular morphology scoring cell number cell viability prostaglandin E2 PGE2 production IL-6 production matrix metalloproteinase 3 MMP3 production